TIM-3基因多态性及慢性HBV感染患者25(OH)D、PDCD4 mRNA、miRNA水平与病情程度的关联  

Association of TIM-3 gene polymorphism,25(OH)D,PDCD4 mRNA ano miRNA levels with disease severity in patients with chronic HBV infection

作　　者：付涵 卢宣霖 叶恩兰 罗敏 冉海敬 FU Han;LU Xuanlin;YE Enlan;LUO Min;RAN Haijing(Chongqing Fuling District Traditional Chinese Medicine Hospital,Chongqing 408000,China;不详)

机构地区：[1]重庆市涪陵区中医院检验科,重庆408000 [2]重庆两江新区人民医院检验科,重庆401147

出　　处：《中华医院感染学杂志》2025年第4期495-500,共6页Chinese Journal of Nosocomiology

基　　金：重庆市医药科技发展计划基金资助项目(2022JH031502)。

摘　　要：目的 探究慢性乙型肝炎病毒(HBV)感染患者25羟维生素D[25(OH)D]、程序性细胞死亡因子4(PDCD4)mRNA、微小核糖核酸(miRNA)水平,T细胞免疫球蛋白黏蛋白分子-3(TIM-3)基因多态性和各指标与病情程度的关联。方法 选取重庆市涪陵区中医院2022年6月-2023年12月收治的149例慢性HBV感染患者为研究组,研究组患者按照病情程度分为慢性乙肝组75例、肝硬化组43例、肝癌组31例;另选取150名健康体检者为健康组。统计研究组、健康组TIM-3基因多态性分布,比较研究组、健康组及不同病情程度组25(OH)D、PDCD4 mRNA、miR-122、miR-155水平,采用Spearman分析25 (OH)D、PDCD4 mRNA、miR-122、miR-155水平与病情程度的相关性。结果 研究组和健康组均符合Hardy-Weinb urg平衡(P=0.281、0.697);研究组TIM-3 rs10053538位点GG基因型与G等位基因频率分别为79.87%与89.60%低于健康组,而GT基因型与T等位基因频率分别为19.46%与10.40%高于健康组(P<0.05)。研究组25(OH)D、PDCD4 mRNA水平分别为(16.62±5.04)ng/ml、(1.63±0.49)低于健康组,miR-122和miR-155水平分别为(6.94±2.01)和(8.47±2.08)高于健康组(P<0.05)。肝硬化组和肝癌组25(OH)D、PDCD4 mRNA水平低于慢性乙肝组,且肝癌组低于肝硬化组,肝硬化组和肝癌组miR-122和miR-155水平高于慢性乙肝组,且肝癌组高于肝硬化组(P<0.05)。25(OH)D、PDCD4 mRNA水平与病情程度呈负相关(r=-0.627、-0.608,P均<0.05);miR-122、miR-155水平与病情严重程度呈正相关(r-0.719、0.652,P均<0.05)。结论 慢性HBV感染患者25 (OH) D、PDCD4mRNA、miR-122、miR-155表达异常,TIM-3rs10053538位点基因多态性与慢性HBV感染患者的易感染性有关。OBJECTIVE To investigate the 25 hydroxyvitamin DL25(OH)DJ,programmed cell death factor 4(PD-CD4)mRNA and micrornas(miR)levels,and T cell immunoglobulin mucin molecule-3(TIM-3)gene polymor-phisms,and the association of each indicator with the severity of the disease in chronic hepatitis B virus(HBV)infected patients.METHODS From Jun.2022 to Dec.2023,149 patients with chronic HBV infection admitted to Chongqing Fuling district traditional Chinese medicine hospital were selected as the study group,the patients in the study group were divided into 75 cases in the chronic hepatitis B group,43 cases in the liver cirrhosis group,and 31 cases in the liver cancer groups according to the degree of disease.Another 150 healthy subjects were se-lected as the control group.The distribution of TIM-3 gene polymorphism in the study group and the control group was described,and the levels of 25(OH)D,PDCD4 mRNA,miR-122 and miR-155 in the study group,the control group and the different disease severity groups were compared.Spearman was used to analyze the correla-tion between the levels of 25(OH)D,PDCD4 mRNA,miR-122,miR-155 and the severity of the disease.RESULTS Hardy-Weinburg equilibrium was met in both study and control groups(P=0.281,0.697).The fre-quencies of GG genotype and G allele at TIM-3 rs10053538 locus in the study group were 79.87%and 89.60%,lower than those in the control group,while the frequencies of GT genotype and T allele were 19.46%and 10.40%(P<0.05),higher than those in the control group.The levels of 25(OH)D and PDCD4 mRNA in the study group were(16.62±5.04)ng/ml and(1.63±0.49),lower than those in the control group,while the lev-els of miR-122 and miR-155 were(6.94±2.01)and(8.47±2.08),higher than those in the control group,re-spectively(P<0.05).The levels of 25(OH)D and PDCD4 mRNA in the liver cirrhosis and liver cancer groups were lower than those in the chronic hepatitis B group,and the liver cancer group were lower than the liver cirrho-sis group.The levels of miR-122 and miR-155 in the liver cirrhosis a

关 键 词：慢性乙型肝炎病毒感染 T细胞免疫球蛋白黏蛋白分子-3 基因多态性 25羟维生素D 程序性细胞死亡因子4 微小核糖核酸 病情程度 关联 

分 类 号：R512.62[医药卫生—内科学]