外周血CD64、CD_(3)^(+)、CD_(4)^(+)、CD_(8)^(+)水平及对早产儿感染的预测价值  

Predictive value of peripheral blood CD64 and CD_(3)^(+),CD_(4)^(+)and CD_(8)^(+)for infection in preterm infants

作　　者：王文英 张佩佩 王莹莹 金闪闪 WANG Wenying;ZHANG Peipei;WANG Yingying;JIN Shanshan(Huaihe Hospital of Henan University,Kaifeng 475000,Henan,China;不详)

机构地区：[1]河南大学淮河医院儿科,河南开封475000 [2]开封市人民医院儿科,河南开封475000

出　　处：《中华医院感染学杂志》2025年第4期593-597,共5页Chinese Journal of Nosocomiology

基　　金：河南省医学科技攻关省部共建重点基金资助项目(SBGJ202102114)。

摘　　要：目的 分析早产儿外周血CD64及CD_(3)^(+)、CD_(4)^(+)、CD_(8)^(+)水平,并分析各指标对早产儿感染的预测价值。方法 选择2020年12月-2023年12月河南大学淮河医院收治的感染早产儿的198例为研究组,另选择同期未感染的200例早产儿为对照组。采用流式细胞仪检测研究组治疗前后及对照组外周血CD64及CD_(3)^(+)、CD_(4)^(+)、CD_(8)^(+)表达水平,采用受试者工作特征(ROC)曲线分析治疗前外周血CD64及CD_(3)^(+)、CD_(4)^(+)水平对早产儿感染的预测价值。结果 研究组外周血中性粒细胞CD64水平为(5.65±1.89)%高于对照组(P<0.05),CD_(3)^(+)、CD_(4)^(+)水平为(50.79±8.45)%、(36.29±9.16)%低于对照组(P<0.05)。全身感染组外周血CD64水平为(6.92±2.04)%高于局部感染组和对照组(P<0.05),CD_(3)^(+)、CD_(4)^(+)水平为(45.47±5.84)%、(29.32±4.55)%低于局部感染组和对照组(P<0.05),且局部感染组外周血CD64水平高于对照组(P<0.05),CD_(3)^(+)、CD_(4)^(+)水平低于对照组(P<0.05);研究组早产儿治疗后外周血CD64水平低于治疗前(P<0.05),CD_(3)^(+)、CD_(4)^(+)水平高于治疗前(P<0.05);CD64、CD_(3)^(+)、CD_(4)^(+)及三者联合诊断单胎早产儿全身感染的AUC分别为0.901、0.918、0.914和0.987,CD64、CD_(3)^(+)、CD_(4)^(+)及三者联合诊断单胎早产儿局部感染的AUC分别为0.847、0.794、0.808和0.937。结论 早产儿感染后,外周血CD64水平升高,CD_(3)^(+)、CD_(4)^(+)水平降低,CD64、CD_(3)^(+)、CD_(4)^(+)联合检测有助于单胎早产儿感染的早期诊断,从而为治疗策略的制定提供指导。OBJECTIVE To analyze the levels of CD64 and CD+,CD4+and CDg+in peripheral blood of preterm infants,and to assess the predictive value of these indicators for infection in preterm infants.METHODS Totally 198 preterm infants with infection admitted to Huaihe Hospital of Henan University from Dec.2020 to Dec.2023 were selected as the study group,and another 2o0 preterm infants without infection during the same period were selected as the control group.The expression levels of CD64,CD_(3)^(+),CD_(4)^(+)and CD_(8)^(+)in peripheral blood before and after treatment in the study group,as well as in the control group were detected by flow cytometry,and the predictive value of CD64,CD_(3)^(+),CD_(4)^(+)and CD_(8)^(+)in peripheral blood neutrophils before treatment for infection in preterm infants was analyzed using receiver operating characteristic(ROC)curve.RESULTS The level of peripheral blood CD64 in the study group was(5.65±1.89)%,higher than that in the control group(P<0.05),while the levels of CD_(3)^(+)and CD_(4)^(+)were(50.79±8.45)%and(36.29±9.16)%,lower than those in the control group(P<0.05).The peripheral CD64 level in the systemic infection group was(6.92±2.04)%,higher than that in the local infection group(P<0.05),while the CD_(3)^(+)and CD_(4)^(+)levelswere(45.47±5.84)% and(29.32±4.55)%,lower than those in the local infection and the control groups(P<0.05),and the CD64 level in peripheral blood in the local infection group was higher than that in the control group(P<0.05),while the levels of CD,+and CD4+were lower than those in the control group(P<0.05).After treatment,the level of peripheral blood CD64 of pre-term infants in the study group was lower than that before treatment(P<0.05),while the levels of CD_(3)^(+)and CD_(4)^(+)were higher than those before treatment(P<0.05).The area under the curves(AUCs)of CD64,CD_(3)^(+)and CD_(4)^(+)and the combination of the three indicators for diagnosing systemic infection in singleton preterm infants were 0.901,0.918,0.914 and 0.987 respectively,and the AUCs of

关 键 词：早产儿 全身感染 局部感染 CD64 CD_(3)^(+) CD_(4)^(+) CD_(8)^(+) 诊断价值 

分 类 号：R722.13[医药卫生—儿科]