电针“风池”“外关”“阳陵泉”对慢性偏头痛模型大鼠痛觉敏化和三叉神经节PKC/TRPV1通路的影响  

Effects of Electroacupuncture at"Fengchi"(GB 20),"Waiguan"(TE 5),and"Yanglingquan"(GB 34)on Nociceptive Sensitization and PKC/TRPV1 Pathway in the Trigeminal Ganglion of Chronic Migraine Model Rats

作　　者：曾翼翔 涂润泽 赵书聪 杨阳 文皓佳 贺卓众 周胜利 谭磊 何可[1] 付磊[1] ZENG Yixiang;TU Runze;ZHAO Shucong;YANG Yang;WEN Haojia;HETAN Lei;HE Ke;FU Lei(The Second Affiliated Hospital of Hunan University of Chinese Medicine,Changsha,410005)

机构地区：[1]湖南中医药大学第二附属医院,湖南省长沙市410005

出　　处：《中医杂志》2025年第3期283-289,共7页Journal of Traditional Chinese Medicine

基　　金：湖南省医学学科青年骨干人才项目(20240304123);湖南省自然科学基金区域联合基金(2023JJ60337);湖南省卫生健康委员会科研重点指导项目(C202303079147);长沙市科学技术局一般项目(kq2208198);长沙市科学技术局指导性科技计划项目(kzd22053);湖南中医药大学研究生创新项目(2023CX54,2024CX059,2024CX060,2024CX064)。

摘　　要：目的 从痛觉敏化角度探讨电针风池、外关、阳陵泉治疗慢性偏头痛的可能作用机制。方法 将40只SPF级SD大鼠随机分为空白组、模型组、电针组、电针+激动剂组、抑制剂组,每组8只。除空白组外,其余各组大鼠采用腹腔注射硝酸甘油建立慢性偏头痛大鼠模型。大鼠造模成功后,电针组予电针双侧“风池”“外关”“阳陵泉”,每次30 min;电针+激动剂组在电针组的基础上经眶下孔注射蛋白激酶C (PKC)激动剂Phorbol 12-myristate 13-acetate Abcam (1.0 ng/μl) 25μl;抑制剂组经眶下孔注射PKC抑制剂Chelerythrine Chloride (1.0 ng/μl) 10μl;空白组、模型组、抑制剂组均予捆绑束缚30 min,不做其他干预。各组均连续干预5天。干预结束后对大鼠进行眶周、后足机械痛阈值及后足热痛阈值测定;分别运用Western Blot法、实时荧光定量PCR法、免疫荧光法检测三叉神经节组织中瞬时受体电位香草酸亚型1 (TRPV1)、磷酸化瞬时受体电位香草酸亚型1 (p-TRPV1)、PKC蛋白水平,Trpv1、Pkc mRNA表达以及TRPV1、PKC平均荧光强度。结果 与空白组比较,模型组大鼠眶周、后足机械痛阈值及后足热痛阈值均降低,三叉神经节组织中TRPV1、PKC、p-TRPV1蛋白水平,Trpv1、Pkc mRNA表达以及TRPV1、PKC平均荧光强度均升高(P<0.05或P<0.01)。与模型组比较,电针组大鼠眶周、后足机械痛阈值及后足热痛阈值均升高,三叉神经节组织中TRPV1、PKC、p-TRPV1蛋白水平,Trpv1、Pkc mRNA表达以及TRPV1平均荧光强度均降低;电针+激动剂组大鼠三叉神经节组织中TRPV1平均荧光强度降低;抑制剂组大鼠眶周机械痛阈值和后足热痛阈值升高,三叉神经节组织中TRPV1、PKC、p-TRPV1蛋白水平以及TRPV1、PKC平均荧光强度均降低(P<0.05或P<0.01)。与电针组比较,电针+激动剂组大鼠三叉神经节组织中TRPV1、PKC、p-TRPV1蛋白水平以及Trpv1 mRNA表达均升高(P<0.05或P<0.01)。结论 电针“风池”�Objective To explore the possible mechanisms of electroacupuncture at Fengchi(GB 20), Waiguan(TE 5), and Yanglingquan(GB 34) in treating chronic migraine from the perspective of nociceptive sensitization.Methods Forty SPF-grade SD rats were randomly divided into blank group, model group, electroacupuncture group, electroacupuncture + agonist group, and inhibitor group, with 8 rats in each group. Except for the blank group, rats were injected intraperitoneally with nitroglycerin to establish a chronic migraine rat model. After successful modeling, the electroacupuncture group received electroacupuncture at bilateral "Fengchi"(GB 20), "Waiguan"(TE 5), and "Yanglingquan"(GB 34) for 30 minutes each session. The electroacupuncture + agonist group received the same electroacupuncture treatment and additional injection of protein kinase C(PKC) agonist Phorbol 12-myristate 13-acetate(1. 0 ng/μl, 25 μl) via the infraorbital foramen. The inhibitor group received PKC inhibitor Chelerythrine Chloride(1. 0 ng/μl, 10 μl) via the infraorbital foramen. The blank group, model group, and inhibitor group underwent restraint for 30 minutes without other interventions. All groups were continuously intervened for 5 days. After the intervention, the nociceptive thresholds(mechanical and thermal pain) of the periorbital area and hind paw were measured. The expression levels of transient receptor potential vanillic acid subtype 1(TRPV1), phosphorylated TRPV1(p-TRPV1), PKC proteins, Trpv1, Pkc mRNA, and the average fluorescence intensity of transient receptor potential vanillic acid subtype 1(TRPV1) and PKC in the trigeminal ganglion were detected using Western Blot, real-time fluorescence quantitative PCR, and immunofluorescence methods. Results Compared with the blank group, the mechanical and thermal pain thresholds of the periorbital area and hind paw were reduced in the model group, and the protein levels of TRPV1, PKC, p-TRPV1, as well as the mRNA expression of Trpv1 and Pkc, and the average fluorescence intensity of TRPV1 and P

关 键 词：慢性偏头痛 痛觉敏化 电针 瞬时受体电位香草酸亚型1 蛋白激酶C 风池 外关 阳陵泉 

分 类 号：R245[医药卫生—针灸推拿学]