水母雪兔子SmGPAT13基因克隆与表达分析  

Cloning and expression analysis of SmGPAT13 gene in Saussurea medusa

作　　者：胡国瑾 段瑞君 HU Guojin;DUAN Ruijun(College of Eco-environmental Engineering,Qinghai University,Xining 810016,China)

机构地区：[1]青海大学生态环境工程学院,西宁810016

出　　处：《青海大学学报》2025年第1期1-7,共7页Journal of Qinghai University

基　　金：青海省中央引导地方科技发展资金计划项目(2023ZY003);生态学世界一流学科研究生科技创新项目(2024-stxy-Y31)。

摘　　要：甘油-3-磷酸酰基转移酶(glycerol-3-phosph ate acyltransferase,GPAT)在植物角质层形成方面具有重要作用。为探究高山植物水母雪兔子(Saussurea medusa)角质层的形成机制,以水母雪兔子为材料,采用RT-PCR技术克隆了与角质层形成相关的GPAT基因,并对其进行生物信息学分析和不同组织(根、茎、叶和花)qRT-PCR的表达量验证。结果表明:克隆得到的SmGPAT13基因含有GPAT家族特征性酰基转移酶结构域(PlsC),cDNA全长序列为1497 bp,可编码498个氨基酸;该蛋白相对分子质量为55.68 kDa,等电点为8.96,蛋白二级结构以α-螺旋和无规则卷曲为主;系统发育树分析表明,SmGPAT13蛋白与菊科小蓬草(Erigeron canadensis)蛋白的亲缘关系最近,且归属于GPAT6亚家族;启动子区顺式作用元件预测发现,SmGPAT13基因的启动子区含有逆境胁迫、生长发育、激素和光响应相关的顺式作用元件;qRT-PCR结果表明,SmGPAT13基因在不同组织中均有表达,且在花中相对表达量最高。本研究可为进一步探究水母雪兔子SmGPAT13基因功能提供理论依据。Glycerol-3-phosphate acyltransferase(GPAT)significantly contributes to the formation of plant cuticle.In order to investig ate the cuticle formation mechanism in the alpine plant Saussurea medusa,the plant was taken as materials to clone GPAT gene associated with cuticle formation using RT-PCR technology.Bioinformatics analysis was performed,and the expression of GPAT gene in various tissues(roots,stems,leaves and flowers)were validated through qRT-PCR.The results are as follows:The cloned SmGPAT13 gene contained the GPAT family characteristic acyltransferase domain(PlsC),with a cDNA full-length sequence of 1497 bp,encoding 498 amino acids;the molecular weight of the protein was 55.68 kDa,and its isoelectric point was 8.96.The secondary structure of the protein was mainly composed ofα-helices and irregular coils.Phylogenetic tree analysis indicated that SmGPAT13 protein was most closely related to Erigeron canadensis protein in the Asteraceae family,and it belonged to the subfamily of GPAT6.The prediction of cis-acting elements in the promoter region revealed that the promoter region of SmGPAT13 gene contained cis-acting elements related to stress,growth and development,hormones and light response.The results of qRT-PCR demonstrated that SmGPAT13 gene was expressed in various tissues and has the highest relative expression level in flowers.This study provides a theoretical basis for further exploring the function of SmGPAT13 gene in Saussurea medusa.

关 键 词：水母雪兔子 SmGPAT13 基因克隆 表达分析 

分 类 号：Q943.2[生物学—植物学]