菊芋抗旱代谢通路挖掘及分析  

Exploration and analysis of drought resistant metabolic pathways in Helianthus tuberosus L.

作　　者：赵孟良 任延靖 ZHAO Mengliang;REN Yanjing(College of Agriculture and Animal Husbandry,Qinghai University,Xining 810016,China;Academy of Agriculture and Forestry Sciences,Qinghai University,Xining 810016,China)

机构地区：[1]青海大学农牧学院,西宁810016 [2]青海大学农林科学院,西宁810016

出　　处：《青海大学学报》2025年第1期47-54,共8页Journal of Qinghai University

基　　金：青海省中央引导地方科技发展基金项目(2024ZY047);2022年度青海省“昆仑英才·高端创新创业人才”项目。

摘　　要：为了明确菊芋抗旱代谢机制相关的关键物质和重要通路,以抗旱菊芋品种青芋2号为研究对象,采用超高效液相色谱(UPLC)和串联质谱(MS/MS)(UPLC-MS/MS),对其在干旱胁迫处理60 h下的主要代谢产物进行了检测与分析。结果表明:在菊芋抗旱代谢产物中获得了160个差异代谢物,其中123个代谢物显著上调表达(P<0.05),37个代谢物显著下调表达(P<0.05)。160个差异代谢物可分为11类,其中氨基酸及其衍生物数量最多,占比为26.3%。通过OPLS-DA分析获得了20个重要代谢物,其中10个代谢物上升,10个代谢物下降。通路富集分析得到干旱响应最显著(P<0.05)的11条通路,说明这11条通路与菊芋的抗旱性密切相关。本研究挖掘出与菊芋抗旱相关的重要代谢通路,可为后续在干旱半干旱等非耕地区推广菊芋种植提供理论基础。In order to clarify the key substances and important pathways related to the drought resistance metabolism mechanism of Helianthus tuberosus L.,this study takes drought resistant variety Qingyu 2 as research object to detect and analyze its main metabolites under drought stress treatment for 60 h,using ultra-high performance liquid chromatography(UPLC)and tandem mass spectrome-try(MS/MS)(UPLC-MS/MS).The results are as follows:A total of 160 differential metabolites were obtained in the drought resistant metabolites of H.tuberosus L.,among which 123 metabolites were significantly up-regulated(P<0.05)and 37 metabolites down-regulated(P<0.05).160 differential metabolites could be divided into 11 categories,among which amino acids and their derivatives had the highest quantity,accounting for 26.3%.A total of 20 important metabolites were obtained through OPLS-DA analysis,with 10 metabolites increased and 10 metabolites decreased.The pathway enrichment analysis identified 11 pathways with the most significant drought response(P<0.05),indicating that these 11 pathways were closely related to the drought resistance of H.tuberosus L.This study identifies important metabolic pathways related to the drought resistance in H.tuberosus L.,which can provide theoretical basis for promoting the cultivation of Helianthus tuberosus L.in the non-cultivated areas such as arid and semi-arid regions in the future.

关 键 词：菊芋 抗旱 代谢组 代谢组标志物 代谢通路 

分 类 号：S632.9[农业科学—蔬菜学]