2018-2024年青岛地区儿童呼吸道感染HAdV流行病学及遗传学特征研究  

A Study on the Epidemiological and Genetic Characteristics of HAdV among Children with Respiratory Infections from 2018 to 2024 in Qingdao,China

作　　者：孙睿 刘子然 刘思彤 梁荣香 朱顺昕 汪照国[1] SUN Rui;LIU Ziran;LIU Sitong;LIANG Rongxiang;ZHU Shunxin;WANG Zhaoguo(Viral Disease Testing Institute,Qingdao Municipal Center for Disease Control and Prevention,Qingdao 266000,China)

机构地区：[1]山东省青岛市疾病预防控制中心病毒性疾病检验所,青岛266000

出　　处：《病毒学报》2025年第1期128-134,共7页Chinese Journal of Virology

基　　金：青岛市科技惠民项目:(项目号:24-1-8-smjk-14-nsh),题目:基于宏基因组学的青岛地区重点呼吸道病毒遗传信息数据库建设及应用研究。

摘　　要：阐明2018-2024年青岛地区儿童呼吸道感染人类腺病毒(Human adenoviruses,HAdVs)流行病学特征及基因特征。采用荧光RT-PCR法对2018-2024年间青岛地区儿童急性呼吸道感染病例咽拭子标本进行HAdV通用型检测,对阳性样本进行hexon基因测序,同时对2023年10月-2024年6月HAdV暴发期间部分阳性样本fiber和penton base基因进行序列测定,采用Mega 11.0软件进行系统发育及分子特征分析。2018年至2023年9月共检出HAdV阳性样本140份,阳性率4.81%(140/2909)。5岁以下婴幼儿是主要感染人群,阳性占比为74.29%(104/140)。主要流行型别为HAdV-B3型(25.81%),HAdV-C2型(22.58%),HAdV-C1型(20.43%)和HAdV-B7型(10.75%)。2023年10月至2024年6月暴发期间共检出HAdV阳性样本133例,阳性率15.52%(133/857),主要流行型别是HAdV-B3型,占比达82.71%,6-12岁儿童为主要感染人群(51.13%)。2018到2023年9月,青岛地区儿童急性呼吸道感染中HAdV主要流行型别以HAdV-B3,HAdV-C1,HAdV-C2型为主,不同年份阳性占比有所不同,5岁以下婴幼儿为主要感染人群。2023年10月至2024年6月间,HAdV-B3暴发流行,6-12岁儿童为主要感染人群。This study aimed to elucidate the epidemiological and genetic characteristics of human adenovirus(HAdV)among children with respiratory tract infections in Qingdao,China from 2018 to 2024.HAdV was detected using fluorescent RT-PCR in throat swab specimens collected from children with acute respiratory infections during this period.Hexon gene sequencing was performed on positive samples,and during the outbreak from October 2023 to June 2024,additional sequencing of fiber and penton base genes was conducted for selected positive samples.Phylogenetic and molecular characteristics were analyzed using Mega 11.0software.From January 2018 to September 2023,140 HAdV positive samples were idetified,with a positive rate of 4.81%(140/2909).Children under 5 years old were the primary affected group,accounting for 74.29%(104/140).The predominant epidemic types were HAdV-B3(25.81%),HAdV-C2(22.58%),HAdV-C1(20.43%),and HAdV-B7(10.75%).During the outbreak from October 2023 to June 2024,133 HAdV-positive samples were identified,with a positivity rate of 15.52%(133/857).The predominant epidemic type was HAdV-B3(82.71%,110/133),and children aged 6-12 years were the primary affected group(51.13%).These findings indicate that from 2018 to September 2023,HAdV-B3 HAdV-C1 and HAdV-C2were the predominant types among children with acute respiratory infection in Qingdao,with annual variations in positivity rates.Children under 5 years old were the primary affected group during this period.However,from October 2023 to June 2024,an outbreak of HAdV-B3 occurred,with children aged 6–12 years being the main affected population.

关 键 词：HAdV 急性呼吸道感染 儿童 流行特征 分型 

分 类 号：R373.9[医药卫生—病原生物学]