一例赛鸽感染鸽Ⅰ型副粘病毒和鸽圆环病毒的诊断、分离鉴定与遗传进化分析  

Diagnosis,Isolation,Identification and Genetic Evolution Analysis of a Mixed Infection Case of PPMV-1 and PiCV

作　　者：徐巧霞 易辰阳 韩雨 姚蓉 胡帅 邓明勇 杨彦伟 金梅林[2] 康超 XU Qiaoxia;YI Chenyang;HAN Yu;YAO Rong;HU Shuai;DENG Mingyong;YANG Yanwei;JIN Meilin;KANG Chao(Wuhan Keqian Biology Co.,Ltd,Wuhan 430070,China;School of Animal Medicine,Huazhong Agricultural University,Wuhan 430070,China)

机构地区：[1]武汉科前生物股份有限公司,武汉430070 [2]华中农业大学动物医学院,武汉430070

出　　处：《病毒学报》2025年第1期166-172,共7页Chinese Journal of Virology

摘　　要：本研究针对四川地区鸽棚发病的赛鸽群开展了实验室诊断和病原的分离鉴定,通过PCR对病料中的病原开展了初步的鉴定,并对鸽I型副粘病毒阳性的组织开展了病原的分离、纯化和遗传进化分析。结果表明,新城疫疫苗免疫的赛鸽群暴发了鸽Ⅰ型副粘病毒、鸽圆环病毒和大肠杆菌的混合感染,死亡率可达30%。从中分离纯化了一株鸽Ⅰ型副粘病毒,对鸡胚有较强的致死率,属于基因Ⅵ型2.1.1.2.2分支,LaSota免疫两次的血清能够部分中和分离的鸽I型副粘病毒的血凝活性,HI平均值为6.40 log2;鸽圆环病毒属于A分支;这两株毒与国内流行的毒株均属于同一进化分支。本研究通过临床诊断和实验室检测对赛鸽混合感染的疫情做出了综合的判定,为鸽棚养殖的疾病监测、疫苗免疫和综合防控提供了参考。This study conducted laboratory diagnosis and pathogen isolation and identification on a group of racing pigeons affected by an outbreak in dovecote in Sichuan area.Preliminary identification of pathogens in the pigeon samples was carried out through PCR,followed by isolation,purification,and genetic evolution analysis of the pathogens from tissues positive of pigeon paramyxovirus 1(PPMV-1).The results revealed a mixed infection of PPMV-1,pigeon circovirus(PiCV),and Escherichia.coli in the racing pigeons,those were immunized with Newcastle disease vaccine(LaSota Strain),with a mortality rate of up to 30%.A wild-type PPMV-1 was isolated and purified,demonstrating high lethality in chicken embryos and belonging to genotype Ⅵ.2.1.1.2.2.Positive serum from pigeons immunized twice with LaSota vaccine partially neutralized the hemagglutination activity of the isolated PPMV-1,with an average HI titer of 6.40 log2.The PiCV belonged to genotype A,while both PPMV-1 and PiCV strains were found to be in the same evolutionary branch as prevalent domestic strains.This study provided a comprehensive assessment of the mixed infection outbreak in racing pigeons through clinical diagnosis and laboratory testing,offering valuable insights for disease monitoring,vaccine immunization,and integrated prevention and control in dovecote.

关 键 词：鸽Ⅰ型副粘病毒 鸽圆环病毒 混合感染 抗体监测 

分 类 号：S855.3[农业科学—临床兽医学]