续筋接骨液对兔肩袖损伤修补术后腱骨愈合的影响及相关机制研究  

Effect and Related Mechanism of Xujin Jiegu Liquid(续筋接骨液)on Tendon-Bone Healing after Rotator Cuff Injury Repair in Rabbits

作　　者：金久楚 唐婕 邝高艳[2] 曹寅生[2] JIN Jiuchu;TANG Jie;KUANG Gaoyan;CAO Yinsheng(Hongdu Hospital of Traditional Chinese Medicine,Nanchang 330038,Jiangxi,China;The First Affiliated Hospital of Hunan University of Chinese Medicine,Changsha 423399,Hunan,China)

机构地区：[1]南昌市洪都中医院,江西南昌330038 [2]湖南中医药大学第一附属医院,湖南长沙423399

出　　处：《辽宁中医杂志》2025年第3期5-10,I0001,共7页Liaoning Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(82274543);湖南省中医药管理局项目(2021088)。

摘　　要：目的观察续筋接骨液对兔肩袖损伤修补术后腱骨愈合的影响。方法用随机数字表法将36只成年健康的新西兰大白兔分为3组,分别为假手术组、实验组以及模型组,每组12只。假手术组只进行皮肤切开后缝合,不进行肩袖损伤造模,用等剂量的0.9%氯化钠溶液灌胃;实验组在造模1周后行肩袖重建手术,用续筋接骨液进行灌胃;模型组在造模1周后行肩袖重建手术并用0.9%氯化钠溶液进行灌胃。疗程均为4周,疗程结束后处死并取材。观察组织学染色,蛋白免疫印迹(Western Blot)检测转化生长因子-β1和血小板衍生生长因子-BB和酶联免疫吸附试验(Enzyme-Linked Immunosorbent Assay,ELISA)检测相关炎症指标。结果(1)组织病理学:假手术组肌腱-骨面连接紧密,界面清楚,肌腱纤维呈轴形整齐排列,纤维束之间的腱细胞规整排列其中,软骨细胞与肌纤维方向一致,整个结构清晰可见,无病理学改变。实验组肌腱-骨面连接紧密,界面较模糊,但依然可见,腱骨结合部有少量炎性细胞,且有可见新生血管生成以及大量的软骨细胞和少量钙化软骨生成。模型组肌腱-骨面结合疏松,肌腱断端间隙少量结合,并且有可见大量炎性细胞,无明显的毛细血管生成,成纤维细胞增多,排列不规则。(2)ELISA检测:模型组和续筋接骨液组中白细胞介素-6(interleukin-6,IL-6)和肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)浓度均高于假手术组,有统计学意义(P<0.05),续筋接骨液组中IL-6和TNF-α浓度均低于模型组,有统计学意义(P<0.05)。(3)Western Blot法检测给药4周后的3组肩袖软骨组织的转化生长因子-β1(transforming growth factor-β1,TGF-β1)、血小板源性生长因子-BB蛋白(platelet-derived growth factor-BB protein,PDGF-BB)的相对表达水平,与假手术组比较,模型组和续筋接骨液组中TGF-β1、PDGF-BB蛋白的表达水平均上调(P<0.01;P<0.05);与模型组比较,续筋接骨液组中TGF-βObjective To observe the effect of Xujin Jiegu Liquid(续筋接骨液)on tendon-bone healing after rotator cuff injury repair surgery in rabbits.Methods Thirty-six adult healthy New Zealand rabbits were divided into three groups by random number table method,which were sham-operated group,experimental group and model group,respectively,12 rabbits in each group.In the sham-operated group,only skin incision and suturing were performed,no rotator cuff injury was modeled,and saline was administered by gavage in equal doses.In the experimental group,rotator cuff reconstruction surgery was performed at 1 week after modeling,and Xujin Jiegu Liquid was administered by gavage.In the model group,rotator cuff reconstruction surgery was performed at 1 week after modeling,and saline was administered by gavage.The duration of treatment was 4 weeks,and the patients were executed and removed at the end of the treatment period.Histological staining and Western Blot were used for detecting transforming growth factor-β1 and platelet-derived growth factor-BB and ELISA for observing the related inflammatory indexes.Results(1)Histopathology:In the sham-operated group(K),the tendon-bone surface was tightly connected,the interface was clear,the tendon fibers were neatly arranged in an axial shape,the tendon cells between the fiber bundles were regularly arranged among them,the chondrocytes were in the same direction as the muscle fibers,the whole structure was clearly visible,and there were no pathological changes.In the experimental group(S),the tendon-bone surface was tightly connected,and the interface was blurred,but still visible.There were a few inflammatory cells in the tendon-bone junction,and there was visible neovascularization,as well as a large number of chondrocytes and a small amount of calcified cartilage production.In the model group(M),the tendon-bone surface was loosely connected,with a small amount of union between the tendon breaks and a large number of inflammatory cells visible,without obvious capillaries,but ther

关 键 词：续筋接骨液 肩袖损伤 腱骨愈合 转化生长因子-Β1 血小板衍生生长因子-BB 

分 类 号：R274[医药卫生—中医骨伤科学]