柴胡疏肝散含药血清的制备及对脂多糖联合干扰素诱导巨噬细胞的抗炎作用  

Anti-inflammation Effects of Chaihu Shugan San(柴胡疏肝散)-Containing Serum on Lipopolysaccharide-and Interferon-induced Macrophage Cell

作　　者：胡海[1] 张珊 卢宇佳 赵静洁[2] HU Hai;ZHANG Shan;LU Yujia;ZHAO Jingjie(Beijing Huguosi Hospital of Traditional Chinese Medicine,Beijing University of Chinese Medicine,Beijing 100035,China;Beijing Friendship Hospital,Capital Medical University,Beijing 100050,China)

机构地区：[1]北京中医药大学附属护国寺中医医院,北京100035 [2]首都医科大学附属北京友谊医院,北京100050

出　　处：《辽宁中医杂志》2025年第3期136-140,I0003,共6页Liaoning Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(82004109)。

摘　　要：目的规范化制备柴胡疏肝散(Chaihu Shugan San,CSS)含药血清,并观察CSS含药血清对脂多糖(lipopolysaccharide,LPS)联合干扰素(interferon-γ,IFN-γ)诱导的小鼠单核巨噬细胞RAW264.7炎症反应的作用,为其抗炎机制的研究提供实验依据。方法SD雄性大鼠灌胃CSS制备含药血清,使用高效液相色谱-质谱联用法检测含药血清中代表性化合物浓度。体外构建LPS联合IFN-γ诱导的巨噬细胞炎症模型,分别加入CSS含药血清或空白大鼠血清,检测巨噬细胞白细胞介素-1β(interleukin-1β,IL-1β)、白细胞介素-6(interleukin-6,IL-6)、白细胞介素-10(interleukin-10,IL-10)、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、精氨酸酶1(arginase 1,ARG1)的mRNA水平,观察CSS含药血清的抗炎作用。结果CSS含药血清中α-香附酮、芍药苷等化合物含量较高。与空白大鼠血清组比较,CSS组可抑制巨噬细胞促炎因子IL-1β、IL-6、TNF-α的mRNA表达,提高抑炎细胞因子IL-10及ARG1的mRNA表达。结论CSS含药血清对LPS联合IFN-γ诱导的巨噬细胞具有一定的抗炎作用。Objective To make the standardization preparation of Chaihu Shugan San(柴胡疏肝散,CSS)-containing serum.To observe the effect of CSS-containing serum on lipopolysaccharide(LPS)combined with interferon(IFN-γ)induced inflammatory response in mouse mononuclear macrophages RAW264.7,and to provide an experimental basis for the study of its anti-inflammatory mechanism.Methods The CSS-containing serum was prepared by gavage in Sprague Dawley(SD)male rats,and the concentrations of representative compounds in the CSS-containing serum were determined by high-performance liquid chromatography-mass spectrometry(HPLC-MS).The anti-inflammatory effects of CSS were observed in an in vitro model of macrophage inflammation induced by LPS combined with IFN-γ.The mRNA levels of interleukin-1β(IL-1β),interleukin-6(IL-6),interleukin-10(IL-10),tumor necrosis factor-α(TNF-α)and arginase 1(ARG1)in macrophages were measured by adding the CSS-containing serum or normal rat serum,respectively.Results The contents of compounds such asα-coumarinone and paeoniflorin were higher in CSS-containing serum group.Compared with those of the CON group,the CSS group inhibited the mRNA expressions of macrophage pro-inflammatory factors IL-1β,IL-6 and TNF-α,and increased the mRNA expressions of anti-inflammatory cytokines IL-10 and ARG1.Conclusion Serum-containing CSS effectively reduces inflammation in LPS combined with IFN-γinduced macrophages.

关 键 词：柴胡疏肝散 含药血清 单核巨噬细胞 抗炎 

分 类 号：R285[医药卫生—中药学]