小麦硝酸盐转运蛋白基因TaNRT2.1.3-B的克隆与表达分析  

Cloning and Expression Analysis of Wheat Nitrate Transporter Gene TaNRT2.1.3-B

作　　者：杨慧玉 房兆峰 柳珊[2] 郝小聪 孙月 李宁 唐益苗[2] Yang Huiyu;Fang Zhaofeng;Liu Shan;Hao Xiaocong;Sun Yue;Li Ning;Tang Yimiao(College of Resources and Environment,Xinjiang Agricultural University,Urumqi,830052;Hybrid Wheat Research Institute,Beijing Academy of Agriculture and Forestry Sciences,Beijing,100097)

机构地区：[1]新疆农业大学资源与环境学院,乌鲁木齐830052 [2]北京市农林科学院杂交小麦研究所,北京100097

出　　处：《分子植物育种》2025年第1期19-26,共8页Molecular Plant Breeding

基　　金：北京市农林科学院协同创新中心建设方案项目(KJCX201907-2);北京市农林科学院科技创新能力建设专项(KJCX20200423);国家自然科学基金项目(31760712)共同资助。

摘　　要：硝酸盐转运蛋白(nitrate transporter, NRT) NRT2.1在植物氮转运过程中起着重要作用。为揭示NRT2.1基因在小麦幼苗期响应氮饥饿中的功能,从小麦中克隆了TaNRT2.1.3-B基因并对其编码蛋白进行了生物信息学分析。结果表明,TaNRT2.1.3-B基因编码区长1 698 bp,编码一个含565个氨基酸的蛋白。进一步预测分析显示,TaNRT2.1.3-B蛋白含有一个保守的MFS_1结构域,定位于细胞质膜;其二级结构由α-螺旋(39.47%)、β-折叠(5.49%)、无规卷曲(40.35%)和延伸链(14.69%)组成。不同物种间NRT2.1蛋白进化分析表明,TaNRT2.1.3-B与硬粒小麦(Triticum turgidum L.)中的同源蛋白亲缘关系最近。利用实时定量PCR对TaNRT2.1.3-B基因在苗期不同组织及在氮饥饿条件下的表达模式进行分析,结果表明,TaNRT2.1.3-B在根中表达量远高于地上部;在氮饥饿条件下,TaNRT2.1.3-B在低氮高效型小麦品种中表达量明显高于低氮低效型小麦品种,推测TaNRT2.1.3-B可能在小麦幼苗响应氮饥饿中发挥着重要作用。本研究结果为小麦NRT2.1基因生物学功能的研究提供了一定的基础,为小麦氮高效种质筛选和培育提供参考。Nitrate transporter(NRT) NRT2.1 plays an important role in the process of plant nitrogen transport. In order to reveal the function of NRT2.1 gene in response to nitrogen starvation in wheat seedling stage, TaNRT2.1.3-B gene was cloned from common wheat, its encoded protein was analyzed by bioinformatics. The results showed that the coding region of TaNRT2.1.3-B gene is 1 698 bp long and encodes a protein containing 565 amino acids. Further prediction analysis showed that TaNRT2.1.3-B protein contains a conserved MFS_1 domain, which is located in the plasma membrane;its secondary structure consists of alpha helix(39.47%), beta turn(5.49%),random coil(40.35%) and extended strand(14.69%). The evolution analysis of NRT2.1 protein among different species showed that TaNRT2.1.3-B has the closest relationship with the homologous protein in durum wheat(Triticum turgidum L.). Using real-time quantitative PCR to analyze the expression pattern of TaNRT2.1.3-B gene in different tissues of seedling stage and under nitrogen starvation conditions. The results showed that the expression of TaNRT2.1.3-B in root was much higher than that in aerial tissue;under the nitrogen starving conditions, the expression level of TaNRT2.1.3-B in low-nitrogen high-efficiency wheat varieties was significantly higher than that of low-nitrogen low-efficiency wheat varieties. It is speculated that TaNRT2.1.3-B may play an important role in the response of nitrogen starvation in wheat seedlings. The results of this study provide a basis for the study of the biological functions of the wheat NRT2.1 gene, and provide a reference for the selection and cultivation of high nitrogen-efficient wheat germplasm.

关 键 词：小麦(Triticum aestivum L.) 硝酸盐转运蛋白 氮饥饿 表达分析 

分 类 号：S512.1[农业科学—作物学]