番茄SlMYB74转录激活分析及互作蛋白的初步筛选  

Transcriptional Activation Analysis and Preliminary Screening of Interaction Proteins of Sl MYB74 from Tomato

作　　者：贾梦玫 郝娟 郭仰东[2] 张喜春[1] Jia Mengmei;Hao Juan;Guo Yangdong;Zhang Xichun(College of Plant Science and Technology,Beijing Agricultural University,Beijing,102206;College of Horticulture,China Agricultural University,Beijing,100094)

机构地区：[1]北京农学院植物科学技术学院,北京102206 [2]中国农业大学园艺学院,北京100094

出　　处：《分子植物育种》2025年第1期27-33,共7页Molecular Plant Breeding

基　　金：北京市教委蔬菜种质资源引进与应用项目(2026016015)资助。

摘　　要：MYB转录因子在植物胁迫应答机制中具有重要的调控作用。本研究利用课题组前期已构建的番茄cDNA文库,采用酵母双杂交技术筛选SlMYB74基因的互作蛋白,进一步探究SlMYB74参与低温胁迫的调控机制。结果显示,本研究构建了诱饵载体pGBKT7-SlMYB74,检测出其无毒性且具有自激活活性;筛选发现3-AT浓度为175 mmol/L时,抑制其自激活效果最好,以此降低试验结果假阳性率;筛库后初步得到9个与Sl MYB74互作的候选蛋白。互作蛋白的GO分析结果表明,MYB74可能参与转录调控、盐胁迫响应、低温响应、抵御昆虫、木质素合成等生物学过程。从中选择SlMYB14、SlMYB60进行点对点验证,结果显示两个蛋白均与SlMYB74相互作用。本研究为进一步探究SlMYB74环境胁迫应答调控机制提供帮助。MYB transcription factors play an important regulatory role in plant stress response mechanism. In this study, the tomato cDNA library previously constructed by our research group was used to screen the interaction proteins of SlMYB74 gene by yeast two-hybrid technology, so as to further explore the regulatory mechanism of SlMYB74 involved in low temperature stress. The bait vector pGBKT7-SlMYB74 was constructed and proved to be nontoxic and self-activated. When the concentration of 3-AT was 175 mmol/L, the inhibition of self-activation was the best, thereby reducing the false positive rate of the test results. Nine candidate proteins interacting with SlMYB74 were preliminarily obtained after screening. GO analysis of the interaction proteins showed that MYB74 may be involved in regulation of transcription, response to salt stress, response to cold, defense response to insect, suberin biosynthetic process and other biological processes. Two candidate proteins, SlMYB14 and Sl MYB60, were selected for point-to-point verification. The results showed that both proteins interacted with Sl MYB74. This study provides help for further exploring the regulation mechanism of environmental stress response of SlMYB74.

关 键 词：番茄 SlMYB74 酵母双杂交 互作蛋白 

分 类 号：S641.2[农业科学—蔬菜学]