西瓜转录因子基因ClERF5的克隆及表达分析  

Cloning and Expression Analysis of Watermelon Transcription Factor Gene ClERF5

作　　者：靳丹丹 张卫华 杨立芳 刘颖 李天杰 刘海学 吴颖 Jin Dandan;Zhang Weihua;Yang Lifang;Liu Ying;Li Tianjie;Liu Haixue;Wu Ying(College of Horticulture and Landscape,Tianjin Agricultural University,Tianjin,300384;School of Chemistry and Chemical Engineering,Guangxi Minzu University,Nanning,530005)

机构地区：[1]天津农学院园艺园林学院,天津300384 [2]广西民族大学化学化工学院,南宁530005

出　　处：《分子植物育种》2025年第1期53-59,共7页Molecular Plant Breeding

基　　金：国家自然科学基金面上项目(61971312);天津市引进领军人才创新类专项;天津市高等学校学科领军人才培养计划项目;天津市研究生科研创新项目(2021YJSS143)共同资助。

摘　　要：AP2/ERF转录因子在植物中广泛存在,其参与植物生长发育及非生物胁迫等一系列生物过程。本研究利用前期西瓜耐旱相关的转录组数据进行筛选,通过RT-PCR技术克隆一个AP2/ERF转录因子基因ClERF5,并对其进行生物信息学分析。结果表明,ClERF5基因CDS序列长度为1014 bp,有完整开放阅读框,编码337个氨基酸,存在保守的AP2/ERF结构域,不存在跨膜结构,内部以无规卷曲和α-螺旋为主,为亲水性蛋白。该转录因子蛋白与苦瓜Mc ERF5蛋白的同源性最高。利用RT-qPCR检测西瓜AP2/ERF在不同组织和干旱胁迫时间下的表达变化,结果显示西瓜ClERF5在不同干旱时间胁迫下均表达上调,并在6 d时达到峰值,表明该基因可能对植物干旱胁迫有响应。组织表达量分析表明,ClERF5在根中的表达量最高。本研究为深入揭示西瓜ClERF5在干旱胁迫应答中的功能及调控机制提供了实验基础。AP2/ERF transcription factors are widespread in plants,and they participate in a series of biological processes such as plant growth and abiotic stress.This study utilized prior transcriptome data related to drought tolerance in watermelon for screening,cloned an AP2/ERF transcription factor gene,ClERF5,using RT-PCR,and then conducted bioinformatic analysis on it.The results showed that the CDS sequence length of ClERF5 gene was1014 bp,contained a complete open reading frame,encoded 337 amino acids,and featured a conserved AP2/ERF domain.ClERF5 protein was hydrophilic protein with no transmembrane structure,and it was dominated by random coils andα-helices.The transcription factor protein had the highest homology with the McERF5 protein of Momordica charantia.RT-qPCR was used to detect the expression changes of watermelon AP2/ERF under different tissues and drought stress times.The results showed that watermelon ClERF5 was up-regulated under different drought stress times,and reached its peak value at 6 days,indicating that this gene might be involved in the response of plants to drought stress.Tissue expression analysis showed that ClERF5 had the highest expression in roots.This study provided an experimental basis for further revealing the function and regulation mechanism of watermelon ClERF5 in response to drought stress.

关 键 词：西瓜 ClERF5转录因子 干旱胁迫 表达分析 

分 类 号：S651[农业科学—果树学]