槟榔响应植原体侵染的转录组分析  

Transcriptome Analysis of Areca cathecu L.Response to Phytoplasmas Infection

作　　者：孟秀利 唐庆华[1] 林兆威 宋薇薇[1] Meng Xiuli;Tang Qinghua;Lin Zhaowei;Song Weiwei(The Innovation Platform for Academicians of Hainan Province,Coconut Research Institute,Chinese Academy of Tropical Agricultural Sciences,Wenchang,571339)

机构地区：[1]中国热带农业科学院椰子研究所,海南省院士创新平台,文昌571339

出　　处：《分子植物育种》2025年第1期202-209,共8页Molecular Plant Breeding

基　　金：海南省自然科学基金项目(321RC659,321QN346);海南省院士创新平台资金项目共同资助。

摘　　要：为了探究槟榔应答植原体侵染的分子机理,本研究对染病槟榔与健康槟榔进行转录组比较分析。通过转录组测序共检测到表达基因24903个,其中355个为可能的抗病基因。染病槟榔与健康槟榔有1816个差异表达基因(differentially expressed genes,DEGs),其中539个DEGs在染病槟榔中上调表达。差异表达基因的GO功能富集分析显示,DNA结合、转录调节活性、DNA结合转录因子活性等13个功能为显著性富集,表明这些功能在槟榔应答植原体侵染过程中发挥特异性作用。通过KEGG信号通路富集分析,富集基因数最多的是植物与病原互作信号通路,共富集了99个DEGs;其次为植物MAPK信号通路,共富集了76个DEGs;再次为植物激素信号转导信号通路,共富集了68个DEGs。说明上述3个信号通路在应答机制中发挥了关键作用。值得注意的是,参与病原与寄主互作的99个DEGs包含29个抗病基因,其中Acat00013480、Acat00031368和Acat00027434上调表达,未来这3个基因可作为潜在抗病基因进一步研究。本研究结果可为研究槟榔与植原体互作分子机理及挖掘槟榔黄化病抗病基因提供参考依据。To explore the molecular mechanism of areca palms response to phytoplasmas infection,we analyzed the transcriptome of diseased areca palms and healthy ones.24903 expressed genes were detected by transcriptome sequencing and 355 ones were probable resistant genes.There were 1816 differentially expressed genes(DEGs)between diseased areca palms and healthy palms.539 DEGs in diseased palms were up-regulated.GO functional enrichment of DEGs showed that 13 terms including DNA binding,transcription regulator activity and DNA-binding transcription factor activity enriched significantly,which indicated that these functions played specific roles in areca palms response to phytoplasmas infection.KEGG pathways enrichment of DEGs showed that plant-pathogen interaction pathway enriched the most DEGs and 99 DEGs were enriched;secondly,MAPK signaling pathway enriched 76 DEGs;thirdly,plant hormone signal transduction pathway enriched 68 DEGs,indicating that these 3pathways played critical roles in the response mechanism.It's worth noting that the 99 DEGs involved in the plant-pathogen interaction pathway include 29 resistant genes and 3 resistant genes including Acat00013480,Acat00031368 and Acat00027434 were up-regulated.These three up-regulated resistant genes can be used as potential r esistant genes for further study.The results provide reference for studying areca palms-phytoplasmas interaction mechanism and exploring YLD resistant genes.

关 键 词：槟榔黄化病 转录组测序 植原体 植物与病原互作 抗病基因 

分 类 号：S763.7[农业科学—森林保护学]