豆科植物红三叶草提取物刺芒柄花素联合运动减轻心梗大鼠心肌损伤的研究  

Effects of Formononetin from Red Clover with Exercise in Reducing Myocardial Injury in Rats with Myocardial Infarction

作　　者：李恒[1] Li Heng(Henan Justice Police Vocational College,Zhengzhou,450018)

机构地区：[1]河南司法警官职业学院,郑州450018

出　　处：《分子植物育种》2025年第1期254-263,共10页Molecular Plant Breeding

摘　　要：本研究旨在评估红三叶草提取物刺芒柄花素(FMN)联合有氧运动对心肌梗死(MI)大鼠心肌损伤的改善作用。通过建立心肌缺血/再灌注损伤(MIRI)模型,探讨FMN不同剂量及其与有氧运动的联合治疗效果。结果显示,FMN与有氧运动联合干预显著改善了心梗大鼠的心功能,提高了左心室短轴缩短率(FS)和射血分数(EF),并显著降低了血清心肌损伤标志物(CK-MB,NT-proBNP,cTnI)水平。同时,联合治疗组在降低心肌组织Fe2+水平、抑制铁死亡相关基因CHAC1的表达、上调抗铁死亡基因(GPX4,SLC7A11,FTH1和FPN1)的表达方面表现出显著的协同效应。这些结果表明,刺芒柄花素与有氧运动的联合应用能够通过多重机制协同减轻心梗大鼠的心肌损伤,为心血管疾病的综合治疗提供了新的实验依据和理论支持。This study aimed to evaluate the effects of red clover extract formononetin(FMN)combined with aerobic exercise on myocardial injury in rats with myocardial infarction(MI).A myocardial ischemia/reperfusion injury(MIRI)model was established to explore the therapeutic effects of different doses of FMN and its combination with aerobic exercise.The results showed that the combined intervention of FMN and aerobic exercise significantly improved cardiac function in MI rats,increasing the left ventricular fractional shortening(FS)and ejection fraction(EF),and significantly reducing serum myocardial injury markers(CK-MB,NT-proBNP,cTnI)levels.Additionally,the combined treatment group exhibited a significant synergistic effect in reducing Fe2+levels in myocardial tissue,inhibiting the expression of the ferroptosis-related gene CHAC1,and upregulating the expression of anti-ferroptosis genes(GPX4,SLC7A11,FTH1,and FPN1).These findings suggest that the combination of formononetin and aerobic exercise can synergistically alleviate myocardial injury in MI rats through multiple mechanisms,providing new experimental evidence and theoretical support for the comprehensive treatment of cardiovascular diseases.

关 键 词：红三叶草(Trifolium pratense) 刺芒柄花素 有氧运动 心肌梗死 铁死亡 心肌损伤 

分 类 号：R285.5[医药卫生—中药学]