基于线粒体ATP6基因和D-loop区序列的密点麻蜥遗传多样性及系统发育分析  

Genetic diversity and phylogenetic analysis of Eremias multiocellata based on mitochondrial ATP6 gene and D-loop zone sequence

作　　者：蔡磊 陈玟含 许猷皇 张俊哲 马钰 李铀 CAI Lei;CHEN Wenhan;XU Youhuang;ZHANG Junzhe;MA Yu;LI You(College of Life Sciences and Engineering,Northwest Minzu University,Lanzhou 730100,China;Gansu Tech-Innovation Centre of Animal Cell,Biomedical Research Centre,Northwest Minzu University,Lanzhou 730030,China)

机构地区：[1]西北民族大学生命科学与工程学院,甘肃兰州730100 [2]西北民族大学生物医学研究中心甘肃省动物细胞技术创新中心,甘肃兰州730030

出　　处：《安徽农学通报》2025年第5期47-52,共6页Anhui Agricultural Science Bulletin

基　　金：国家自然科学基金地区基金项目(32060311);中共甘肃省委组织部省级人才项目(51202506);兰州市城关区科技局科技人才创新创业项目(2023-rc-6);西北民族大学中央高校基本科研业务费项目(31920240109)。

摘　　要：为探讨西北部分地区密点麻蜥的遗传多样性和系统发育关系,以甘肃、宁夏和内蒙古的8个采样区域的密点麻蜥线粒体ATP6基因和线粒体控制区(D-loop)序列为分子标记进行PCR扩增并送样测序,利用MEGA-X和DnaSP 6.1.2软件分析不同种群密点麻蜥ATP6基因和D-loop区序列以及遗传多样性,以最大邻接法(NJ)和最大似然法(ML)分别构建NJ树和ML树,探讨不同密点麻蜥种群之间的系统发育关系。结果表明,PCR扩增ATP6基因和D-loop目的条带大小分别在400~600 bp和650~800 bp,条带清晰符合预期;序列分析表明,ATP6基因序列的A+T=55.6%,C+G=44.4%,D-loop区序列的A+T=61.7%,C+G=38.3%,碱基组成具有偏倚性。遗传多样性结果表明,宁夏陶乐地区密点麻蜥的ATP6基因单倍型数量(h)为8个,单倍型多样性(Hd)为0.933,核苷酸多样性(π)为0.013 78;D-loop在该地区h为6个,Hd为0.844,π为0.007 77,该区密点麻蜥种群的遗传变异程度较高。系统发育分析表明,在甘肃、宁夏和内蒙古的8个采样区域的密点麻蜥种群中均存在一定程度的基因交流。研究结果为密点麻蜥种质资源的保护和开发提供参考。To investigate the genetic diversity and phylogenetic relationship of Eremias multiocellata in some areas of Northwest China,mitochondrial ATP6 gene and mitochondrial control region(D-loop)sequence of 8 sampling areas in Gansu,Ningxia and Inner Mongolia were amplified by PCR and sent for sample sequencing.MEGA-X and DnaSP 6.1.2 software were used to analyze the ATP6 gene and D-loop zone sequences and genetic diversity of different populations of Eremias multiocellata.NJ and ML trees were constructed by maximum adjacency method(NJ)and maximum likelihood method(ML),respectively,to explore the phylogenetic relationships among different populations of Eremias multiocellata.The results showed that the bands of ATP6 gene and D-loop amplified by PCR were 400-600 bp and 650-800 bp respectively,and the bands were clear and consistent with expectations.Sequence analysis showed that A+T=55.6%,C+G=44.4%in ATP6 gene sequence,A+T=61.7%,C+G=38.3%in D-loop zone sequence,with base composition bias.The genetic diversity results showed that the number(h)of ATP6 gene was 8,the haplotype diversity(Hd)was 0.933,and the nucleotide diversity(π)was 0.01378.D-loops h was 6,Hd was 0.844,πwas 0.00777 in this area.The genetic variation of the population was higher in this area.Phylogenetic analysis showed that there were different degrees of gene exchange in 8 populations of Eremias multiocellatain in Gansu,Ningxia and Inner Mongolia.The results of this study provide a reference for the conservation and development of the germplasm resources of Eremias multiocellata.

关 键 词：密点麻蜥 线粒体DNA 系统发育分析 遗传多样性 

分 类 号：S813.24[农业科学—畜牧学]