基于斑马鱼模型的人参阿胶口服液对气血及免疫功能调节作用评估  

Evaluate the regulatory effects of ginseng donkey-hide gelatin solution on qi,blood and immune function based on zebrafish model

作　　者：韩亮 裴庆华 李莉 曾傲琼 HAN Liang;PEI Qinghua;LI Li;ZENG Aoqiong(Shandong Gubentang Health Industry Development Group Co.,Ltd.,Jinan 250000,China;Jiangnan University,Wuxi 214000,China)

机构地区：[1]山东固本堂健康产业开发集团股份有限公司,山东济南250000 [2]江南大学,江苏无锡214000

出　　处：《安徽农学通报》2025年第5期107-113,共7页Anhui Agricultural Science Bulletin

摘　　要：为研究人参阿胶口服液对气血及免疫功能的调节作用,以受精后3 d(3 dpf)的野生型AB品系斑马鱼为试验对象,分别通过苯肼和酒石酸长春瑞滨诱导建立斑马鱼贫血和免疫力低下模型,分为正常对照组、模型对照组和人参阿胶口服液组,测定人参阿胶口服液的最大耐受浓度(MTC);通过MTC和斑马鱼贫血及免疫力低下模型将试验分为正常对照组、模型对照组、阳性对照组和人参阿胶口服液组,评估其调节气血和免疫力的效果。结果表明,人参阿胶口服液调节气血能力的MTC为31.20μL/mL;与模型对照组相比,在浓度为15.60和31.20μL/mL的处理组中,斑马鱼的心脏红细胞染色强度分别增加了276.64%和570.47%。人参阿胶口服液调节免疫力功能的MTC为3.91μL/mL,与模型对照组相比,在0.98、1.95和3.91μL/mL的处理组中,斑马鱼体内残留荧光颗粒数量分别减少了34.35%、40.42%和52.10%;此外,在浓度为1.95和3.91μL/mL的处理组中,斑马鱼体内T细胞荧光强度明显提高,分别比模型对照组增加了110.24%和126.94%。综上,人参阿胶口服液具有改善贫血的效果,且能通过促进巨噬细胞吞噬和增强T细胞功能来调节免疫力。To investigate the regulating effect of ginseng donkey-hide gelatin solution on qi,blood and immune function.The wild type AB strain zebrafish 3 dpf after insemination was used as the experimental object.The anemia and immune deficiency models of zebrafish were induced by phenylzine and vinorelbine tartrate,respectively,and were divided into normal control group,model control group and ginseng donkey-hide gelatin solution group.The maximum tolerance concentration(MTC)of ginseng donkey-hide gelatin solution was determined.The experiment was divided into normal control group,model control group,positive control group and ginseng donkey-hide gelatin solution group by MTC and zebrafish anemia and low immunity model to evaluate the effect of regulate qi,blood and immunity.The results showed that the MTC of ginseng donkey-hide gelatin solution to regulate the ability of qi,blood was 31.20µL/mL.Compared with the model control group,in the treatment group of 15.60 and 31.20µL/mL,the cardiac erythrocyte staining intensity of zebrafish increased by 276.64%and 570.47%,respectively.The MTC of ginseng donkey-hide gelatin solution regulating immune function was 3.91µL/mL.Compared with the model control group,the number of residual fluorescent particles in zebrafish in 0.98,1.95,and 3.91µL/mL treatment groups decreased by 34.35%,40.42%,and 52.10%,respectively.In addition,in the 1.95 and 3.91µL/mL treatment groups,the fluorescence intensity of T cells in zebrafish was significantly increased,which was 110.24%and 126.94%higher than that in the model control group,respectively.To sum up,ginseng donkey-hide gelatin solution has the effect of improving anemia,and can regulating immunity by promoting macrophage phagocytosis and enhancing T cell function.

关 键 词：人参阿胶口服液 斑马鱼 贫血 免疫调节 

分 类 号：R285.5[医药卫生—中药学]