章鱼胺、多巴胺对桔小实蝇嗅觉学习和记忆的差异性调控研究  

The differential regulation of octopamine and dopamine on olfactory learning and memory in Bactrocera dorsalis

作　　者：张学风 林涛[2] 林小军 瞿佳宝 项前 曾鑫年[1] 刘家莉[1] ZHANG Xue-Feng;LIN Tao;LIN Xiao-Jun;QU Jia-Bao;XIANG Qian;ZENG Xin-Nian;LIU Jia-Li(Guangdong Engineering Research Center for Insect Behavior Regulation,South China Agricultural University,Guangzhou 510642,China;College of Life Science,Shangrao Normal University,Shangrao 334001,Jiangxi Province,China;Guangzhou Ruifeng Biotechnology Co.,Ltd.,Guangzhou 510663,China)

机构地区：[1]华南农业大学植物保护学院,广东省昆虫行为调控工程技术研究中心,广州510642 [2]上饶师范学院生命科学学院,江西上饶334001 [3]广州瑞丰生物科技有限公司,广州510642

出　　处：《环境昆虫学报》2025年第1期128-139,共12页Journal of Environmental Entomology

基　　金：国家自然科学基金(32072486);国家重点研发计划(2021YFD1000503-03)。

摘　　要：章鱼胺(Octopamine,OA)和多巴胺(Dopamine,DA)作为普遍存在于昆虫体内的生物胺,参与调控其学习和记忆行为。OA以及DA通过与相应的章鱼胺受体(Octopamine receptors,OARs)或多巴胺受体(Dopamine receptors,DARs)专一性结合,进而对昆虫的生理过程产生调控效果。本研究旨在通过药理干涉的方法来探索OA和DA对桔小实蝇Bactrocera dorsalis嗅觉学习和记忆提取的差异性调控作用。结果表明:在桔小实蝇的学习形成中,注射2种OARs拮抗剂酚妥拉明、育亨宾可显著降低其奖赏性嗅觉学习,但不影响其惩罚性嗅觉学习,且两种拮抗剂的效应受到注射浓度和时间的影响。注射2种DARs拮抗剂SCH23390和螺哌隆可显著降低其惩罚性嗅觉学习,但不影响其奖赏性嗅觉学习,2种拮抗剂的效果主要受到注射浓度的影响。在桔小实蝇的记忆提取中,发现注射2种OARs拮抗剂可显著降低桔小实蝇奖赏性记忆的提取能力,但不影响其惩罚性记忆的提取,酚妥拉明的效果受到注射时间的影响,而育亨宾的效果受到注射浓度的影响。注射DARs可同时降低桔小实蝇奖赏性和惩罚性记忆的提取,其中注射SCH23390主要降低惩罚性嗅觉记忆的提取能力,而螺哌隆主要降低奖赏性嗅觉记忆的提取能力。以上结果显示,OA和DA在调控桔小实蝇的嗅觉学习和记忆提取中存在差异性,研究结果可为靶向昆虫学习的行为调节剂提供理论参考。Octopamine(OA)and Dopamine(DA)are biological amines commonly found in insects and are involved in regulating learning and memory behaviors.OA and DA regulate insect physiological processes by binding specifically to their corresponding receptors,Octopamine receptors(OARs)and Dopamine receptors(DARs),respectively.The purpose of this study was to explore the differential regulatory effects of OA and DA on olfactory learning and memory retrieval in Bactrocera dorsalis through pharmacological intervention.The results showed that the injection of two OAR antagonists,phentolamine and yohimbine,significantly reduced reward-based olfactory learning in B.dorsalis but did not affect punishment-based olfactory learning.The effects of these antagonists were influenced by both injection concentration and time.In contrast,the injection of two DAR antagonists,SCH23390 and spiperone,significantly reduced punishment-based olfactory learning but had no effect on reward-based olfactory learning.The effects of these DAR antagonists were primarily influenced by injection concentration.In memory retrieval experiments,the injection of OAR antagonists significantly impaired the retrieval of reward-based memories but did not affect punishment-based memories.The effect of phentolamine was dependent on injection time,while the effect of yohimbine was dependent on injection concentration.On the other hand,the injection of DAR antagonists impaired the retrieval of both reward and punishment memories.Specifically,SCH23390 mainly reduced the retrieval of punishment-based olfactory memories,while spiperone primarily reduced the retrieval of reward-based olfactory memories.These results indicate that OA and DA play distinct roles in regulating olfactory learning and memory retrieval in B.dorsalis.The findings provide a theoretical foundation for developing behavior regulators targeting insect learning processes.

关 键 词：桔小实蝇 章鱼胺 多巴胺 受体 嗅觉学习与记忆 

分 类 号：Q968.1[生物学—昆虫学]