微小RNA-299-3p通过磷脂酰肌醇3激酶/蛋白激酶B通路调控鼻咽癌增殖、侵袭及迁移的研究  

MicroRNA-299-3p regulates proliferation,invasion and migration of nasopharyngeal carcinoma through phosphatidylinositol 3-kinase/protein kinase B pathway

作　　者：穆热迪力·穆塔里甫 阿卜拉·艾则孜 皮力东·库亚西[1] 张维娜 雍军[1] Muredili MUTALIFU;Abula AIZEZI;Pilidong KUYAXI;ZHANG Weina;YONG Jun(Department of Otolaryngology,the First Affiliated Hospital of Xinjiang Medical University,Urumqi,Xinjiang,830054)

机构地区：[1]新疆医科大学第一附属医院耳鼻喉科,新疆乌鲁木齐830054

出　　处：《实用临床医药杂志》2025年第3期6-10,16,共6页Journal of Clinical Medicine in Practice

基　　金：新疆维吾尔自治区自然科学基金项目(2021D01C320)。

摘　　要：目的探讨微小RNA(miR)-299-3p通过磷脂酰肌醇3激酶(PI3K)/蛋白激酶B(AKT)信号通路调控鼻咽癌细胞增殖、侵袭及迁移的作用。方法根据miR-299-3p表达水平,将人鼻咽癌细胞系CNE2分为对照组(miR-299-3p不表达)、过表达组(miR-299-3p过表达)和低表达组(miR-299-3p低表达)。采用CCK-8实验检测细胞增殖率,流式细胞术检测凋亡率,Transwell小室检测细胞侵袭和迁移情况,Western blot检测磷酸化PI3K(p-PI3K)和磷酸化AKT(p-AKT)蛋白表达,实时荧光定量聚合酶链式反应检测miR-299-3p表达情况。结果与对照组比较,过表达组细胞增殖率、侵袭和迁移细胞数量减少,凋亡率增加,差异有统计学意义(P<<0.05);与对照组比较,低表达组细胞增殖率、侵袭和迁移数量、p-PI3K和p-AKT蛋白表达量增加,凋亡率下降,差异有统计学意义(P<0.05)。结论miR-299-3p过表达可能通过抑制PI3K/AKT信号通路抑制鼻咽癌细胞增殖、侵袭和迁移行为。Objective To investigate the role of microRNA(miR)-299-3p in regulating the proliferation,invasion,and migration of nasopharyngeal carcinoma cells through phosphatidylinositol 3-kinase(PI3K)/protein kinase B(AKT)signaling pathway.Methods The human nasopharyngeal carcinoma cell line CNE2 was randomly divided into three groups:control group(no expression of miR-299-3p),over-expression group overexpression of miR-299-3p,and miR-299-3p low-expression group(low expression of miR-299-3p).CCK-8 experiment was to detect cell proliferation rate,flow cytometry was to detect apoptosis rate,Transwell chamber was to detect invasion and migration,Western blot was to detect p-PI3K and p-AKT protein expressions,real-time fluorescence quantitative polymerase chain reaction was to detect miR-299-3p expressions.Results Compared with the control group,the over-expression group showed significant decreases in cell proliferation rate,the number of cells with invasion and migration,increase in apoptosis rate;compared with the control group,the low-expression group showed significant increases in cell proliferation rate,the number of cells with invasion and migration,phosphorylated PI3K(p-PI3K)and phosphorylated AKT(p-AKT)protein expression,decrease in apoptosis rate(P<0.05).Conclusion Over-expression of miR-299-3p may affect the proliferation,invasion,and migration behaviors of nasopharyngeal carcinoma cells by inhibiting PI3K/AKT signaling pathway activity.

关 键 词：鼻咽癌 微小RNA-299-3p 磷脂酰肌醇3激酶 蛋白激酶B 

分 类 号：R762[医药卫生—耳鼻咽喉科] R73[医药卫生—临床医学] R739.6