咽拭子7项呼吸道病原体核酸双扩增法在儿童急性上呼吸道感染诊断中的应用价值  

Application value of dual amplification method for nucleic acid detection of seven respiratory pathogens by throat swab samples in diagnosis of acute upper respiratory tract infections in children

作　　者：周永清 王江荷 林亨演 杨凯琪 饶楠 王曼 褚红娟 ZHOU Yongqing;WANG Jianghe;LIN Hengyan;YANG Kaiqi;RAO Nan;WANG Man;CHU Hongjuan(Department of Pathology,Hainan Hospital of Shanghai Children′s Medical Center Affiliated to Medical College of Shanghai Jiaotong University,Sanya,Hainan,572000)

机构地区：[1]上海交通大学医学院附属上海儿童医学中心海南医院病理科,海南三亚572000

出　　处：《实用临床医药杂志》2025年第3期114-117,共4页Journal of Clinical Medicine in Practice

基　　金：上海儿童医学中心三亚市妇女儿童医院金椰种子基金项目(JYZZ-YJ-07)。

摘　　要：目的比较不同检测方法诊断儿童急性呼吸道感染病原体的价值。方法纳入862例急性呼吸道感染患儿,应用双扩增法及自建核酸检测系统对患儿的咽拭子样本进行7种常见的呼吸道病原体的核酸检测。对于2种方法检测结果不一致的样本,通过巢式聚合酶链反应(PCR)进行检测。结果双扩增法阳性检出率为57.75%,高于自建核酸检测系统的30.14%,复合感染检出率为10.14%,高于自建核酸检测系统1.97%,差异有统计学意义(P<0.05)。双扩增法灵敏度为91.63%,高于自建核酸检测系统72.61%,特异性为92.31%,高于自建核酸检测系统75.62%,差异有统计学意义(P<0.05)。结论双扩增法可同时完成7种呼吸道病原体的核糖核酸检测,敏感性高,特异性强,具有较高的临床应用价值。Objective To compare the diagnostic values of different detection methods for respiratory pathogens in children with acute respiratory infections.Methods A total of 862 children with acute respiratory infections were enrolled,and their throat swab samples were tested for seven common respiratory pathogens by the dual amplification method and a self-built nucleic acid detection system.For samples with inconsistent results between the two methods,nested polymerase chain reaction(PCR)was performed for verification.Results The positive detection rate of the dual amplification method was 57.75%,which was significantly higher than 30.14%of the self-built nucleic acid detection system,and the detection rate of mixed infections was 10.14%,which was also significantly higher than 1.97%of the self-built nucleic acid detection system(P<0.05).The sensitivity of the dual amplification method was 91.63%,which was significantly higher than 72.61%of the self-built nucleic acid detection system,and the specificity was 92.31%,which was also significantly higher than 75.62%of the self-built nucleic acid detection system(P<0.05).Conclusion The dual amplification method can simultaneously detect the ribonucleic acid of seven respiratory pathogens with high sensitivity and specificity,demonstrating significant clinical application value.

关 键 词：咽拭子 呼吸道病原体 双扩增法 自建核酸检测系统 

分 类 号：R725.6[医药卫生—儿科] R372[医药卫生—临床医学] R446.1