正交实验设计优化及己多糖提取工艺  

Optimization of Chloranthus serratus polysaccharides extraction technology by orthogonal experimental design

作　　者：陈彩云 谢先进 李安琪 檀圆圆 丁浩 方源孝 李晨曦 孙淑萍 CHEN Caiyun;XIE Xianjin;LI Anqi;TAN Yuanyuan;DING Hao;FANG Yuanxiao;LI Chenxi;SUN Shuping(School of Pharmacy,Wannan Medical College,Wuhu 241002,Anhui,China;Graduate School,Wannan Medical College,Wuhu 241002,Anhui,China;Institute of Natural Daily Chemicals,Wannan Medical College,Wuhu 241002,Anhui,China)

机构地区：[1]皖南医学院药学院,安徽芜湖241002 [2]皖南医学院研究生学院,安徽芜湖241002 [3]皖南医学院天然日化研究所,安徽芜湖241002

出　　处：《右江民族医学院学报》2025年第1期65-70,97,共7页Journal of Youjiang Medical University for Nationalities

基　　金：安徽省教育厅重点教研项目(2023jyxm0655);安徽省研究生创新创业实践项目(2022cxcysj181);皖南医学院重点教研项目(2022jyxm06)。

摘　　要：目的探讨正交实验优化及己的多糖提取工艺。方法采用水提醇沉法提取及己多糖。多糖含量的测定采用苯酚-硫酸法,利用单因素及正交实验优化及己多糖提取的料液比、温度、时间和次数,以精密度、重复性、稳定性、加样回收率多个方法学实验,验证多糖含量测定方法的可行性。通过单因素实验优化及己多糖的醇沉时间及醇沉浓度。结果优化出及己多糖的最佳水提工艺条件为:提取料液比1∶30,提取温度100℃,提取3次,提取时间1.5 h。确定及己多糖的最优醇沉条件为75%乙醇浓度,醇沉后静置18 h。结论本研究提取工艺优化合理,能最大程度提高及己多糖的提取率,为进一步分离纯化及己多糖奠定基础。Objective To optimize the extraction technology of Chloranthus serratus(C.serratus)polysaccharides by orthogonal experimental design.Methods The polysaccharides of C.serratus were extracted by the method of water extraction and alcohol precipitation.The polysaccharides contents were determined by phenol-sulfuric acid method.The solid-liquid ratio,temperature,time,and number of extractions for the extraction of C.serratus polysaccharides were optimized through single-factor and orthogonal experiments.The feasibility of the polysaccharide content determination method was verified through precision,reproducibility,stability,and sample recovery rate experiments.The alcohol precipitation time and concentration for C.serratus were optimized through single-factor experiments.Results The optimum water extraction conditions of C.serratus polysaccharides were determined as follows:the solid-liquid ratio was 1∶30,the extraction temperature was 100℃,extracted for 3 times,the extraction time was 1.5 h.The optimal conditions for alcohol precipitation of C.serratus polysaccharides were determined to be a 75%ethanol concentration,followed by static precipitation for 18 hours.Conclusion The extraction process optimization in this study is reasonable and can maximize the extraction rate of C.serratus polysaccharides,laying a foundation for further isolation and purification of C.serratus polysaccharides.

关 键 词：及己多糖 水提醇沉 单因素实验 正交实验 工艺优化 

分 类 号：R343[医药卫生—基础医学]