四川省泸州市一起霍乱疫情调查及病原特征分析  

Investigation of a cholera epidemic and pathogen characterization in Luzhou,Sichuan province

作　　者：姜敏 毛亚 刘小红 李丹 雷高鹏[2] 许文平[1] 余光才 JIANG Min;MAO Ya;LIU Xiaohong;LI Dan;LEI Gaopeng;XU Wenping;YU Guangcai(Luzhou Center for Disease Control and Prevention,Luzhou,Sichuan 646300,China;不详)

机构地区：[1]泸州市疾病预防控制中心,四川泸州646300 [2]四川省疾病预防控制中心 [3]泸州市江阳区疾病预防控制中心

出　　处：《中国国境卫生检疫杂志》2025年第1期38-41,69,共5页Chinese Journal of Frontier Health and Quarantine

摘　　要：目的调查2022年泸州市一起霍乱疫情,了解霍乱弧菌菌株的耐药性及基因组特征。方法采集病例及其家属、同餐人员、病例居住环境、水产市场环境、水产品和生活饮用水等267份样本,进行霍乱弧菌分离培养,并对分离的霍乱弧菌进行血清学分型和霍乱毒素基因(ctxAB)鉴定。根据国家致病菌识别网(China PIN)指南进行药敏试验,利用二代测序平台分析毒力基因、耐药基因预测及系统进化。结果从267份样本中分离到7株O139群霍乱弧菌,分别来源于病例1株、环境1株和健康带菌者5株,所有菌株对多黏菌素E、四环素、萘啶酸和链霉素耐药,对氯霉素中介;测序显示分离菌株均携带对氟喹诺酮类、多肽类、磺胺类、四环素类、β-内酰胺类、大环内酯类、氨基糖苷类和氯霉素类耐药的相关基因及ctxAB、hlyA等毒力基因;多位点序列分型(MLST)显示所有菌株为ST69型,核心基因组多位点序列分型(cgMLST)为cgST-1314,病例分离株(SC2022LZS511)与2022年陕西省的霍乱病例分离株只相差4个单核苷酸多态性(SNP)位点差异。结论导致本起疫情的O139群霍乱弧菌为国内流行菌株,对多种抗生素耐药,携带多种耐药基因和毒力基因.Objective To investigate a cholera outbreak in Luzhou City,China in 2022,and to identify the drug resistance and genomic characteristics of Vibrio cholerae strains.Methods A total of 267 samples were collected from cholera cases,their family members and meal companions,the living environment of these cases,the environment of aquatic products market,aquatic products,and domestic drinking water for V.cholerae isolation and culture.The isolates were subjected to serological typing and cholera toxin gene(ctxAB)identification.Drug susceptibility test was performed according to the guideline of Chinese Pathogen Identification Net,and next generation sequencing was used for virulence gene analysis,resistance gene prediction,and phylogenetic analysis.Results Seven isolates of V.cholerae serogroup O139 were obtained from the 267 samples,including one from a case,one from the environment and five from healthy carriers.All the isolates were resistant to polymyxin E,tetracycline,nalidixic acid and streptomycin,but showed intermediate resistance to chloramphenicol.Sequencing revealed that all the isolates carried genes related to resistance to fluoroquinolones,polypeptides,sulfonamides,tetracyclines,β-lactams,macrolides,aminoglycosides and chloramphenicol,as well as virulence genes such as ctxAB and hlyA.All the isolates were ST69 according to multilocus sequence typing analysis and cgST-1314 according to core-genome multilocus sequence typing.The isolate from the case(SC2022LZS511)differed by only four single nucleotide polymorphism sites from the cholera case isolate from Shaanxi Province in 2022.Conclusion The V.cholerae serogroup O139,which caused this outbreak,was a domestically circulating strain that exhibited resistance to a variety of antibiotics and carries multiple resistance and virulence genes.

关 键 词：霍乱弧菌 O139血清群 核心基因组单核苷酸变异 耐药性 毒力基因 

分 类 号：R183.4[医药卫生—流行病学] R516.55[医药卫生—公共卫生与预防医学]