北方灵芝属栽培种质遗传多样性评价研究  

Evaluation of the Genetic Diversity of Cultivated Germplasm of Ganoderma Genus in North China

作　　者：王鹏 闫淇 王飞 温嘉伟[1] 李海燕[3] 于娅[1] WANG Peng;YAN Qi;WANG Fei;WEN Jiawei;LI Haiyan;YU Ya(Economic Plants Research Institute,Jilin Academy of Agricultural Sciences(Northeast Agricultural Research Center of China),Changchun,Jilin 136105;College of Horticulture,Jilin Agricultural University,Changchun,Jilin 130118;Institute of Agro-food Technology,Jilin Academy of Agricultural Sciences(Northeast Agricultural Research Center of China),Changchun,Jilin 130033)

机构地区：[1]吉林省农业科学院(中国农业科技东北创新中心)经济植物研究所,吉林长春136105 [2]吉林农业大学园艺学院,吉林长春130118 [3]吉林省农业科学院(中国农业科技东北创新中心)农产品加工研究所,吉林长春130033

出　　处：《北方园艺》2025年第5期16-24,共9页Northern Horticulture

基　　金：吉林省科技发展计划资助项目(20210202074NC);吉林省重要食药用菌关键核心技术示范推广资助项目(202400601);吉林省农业科技创新工程人才基金资助项目(2060302)。

摘　　要：以来自黑龙江、吉林、辽宁、北京、山东等地39株灵芝属菌株为试材,采用ITS序列测定、体细胞不亲和性试验和SRAP分子标记方法进行遗传多样性分析,确定其种间划分及系统发育关系,以期为我国北方地区灵芝属栽培种质的分类、亲缘关系和遗传背景提供参考依据。结果表明:供试的39个灵芝属菌株包括赤芝Ganoderma lingzhi、紫芝G.sinense、松杉灵芝G.tsugae、无柄灵芝G.resinaceum和有柄灵芝G.gibbosum 5个种,相互之间有拮抗反应共713对,无拮抗反应28对,遗传相似系数(GS)为0.50~0.93,平均值为0.69。综上可知,我国北方灵芝属栽培种质存在种类丰富,遗传背景差异大,遗传多样性强的特征,但部分栽培菌株之间无拮抗反应且遗传距离很近,存在着同物异名现象。Taking 39 strains of Ganoderma species from Heilongjiang,Jilin,Liaoning,Beijing,Shandong and other places as experimental materials,the genetic diversity was analyzed by ITS sequence determination,somatic cell incompatibility test and SRAP molecular marker method to determine the species classification and systematic relationship,in order to provide reference for the classification,genetic relationship and genetic background of Ganoderma cultivar resources in northern China.The results showed that the 39 strains of Ganoderma genus tested included 5 species,Ganoderma lingzhi,G.sinense,G.tsugae,G.resinacium and G.gibbosum,and there were 713 pairs of antagonistic reactions and 28 pairs of non-antagonistic reactions among 39 strains,and the genetic similarity coefficient (GS) of the tested strains ranged from 0.50 to 0.93,the average value was 0.69.In conclusion, Ganoderma genus germplasm resources in northern China had significant differences in genetic background and strong genetic diversity,but there were also synonymous phenomena.

关 键 词：灵芝属 分子标记 体细胞不亲和 聚类分析 

分 类 号：S567.3[农业科学—中草药栽培]