野生型和发根转化Gshdz4红车轴草耐盐碱性分析  

Analysis of Salt-alkali Tolerance of Wild Type and Hair Root Gshdz4 Transformation in Trifolium pratense

作　　者：唐继祥 张俊峰 刘梦雨 刘禹杭 李燕萍 曹蕾 TANG Jixiang;ZHANG Junfeng;LIU Mengyu;LIU Yuhang;LI Yanping;CAO Lei(College of Horticulture and Landscape Architecture,Northeast Agricultural University,Harbin,Heilongjiang 150030;College of Geography and Tourism,Harbin University,Harbin,Heilongjiang 150030)

机构地区：[1]东北农业大学园艺园林学院,黑龙江哈尔滨150030 [2]哈尔滨学院地理与旅游学院,黑龙江哈尔滨150030

出　　处：《北方园艺》2025年第5期74-82,共9页Northern Horticulture

基　　金：黑龙江省自然科学基金资助项目(LH2021C058);哈尔滨学院青年博士科研启动基金资助项目(HUDF2019104);黑龙江省大学生创新训练资助项目(202210234027);国家自然青年科学基金资助项目(32001505)。

摘　　要：以红车轴草为试材,利用40、80、120、160、320 mmol·L^(-1) NaCl溶液和20、40、60、80、160 mmol·L^(-1) NaHCO 3溶液分别对红车轴草种子和幼苗进行处理,研究了盐碱胁迫下红车轴草种子的发芽势、根长等生长指标,观察胁迫下0、4、8、20、24、32、44、48 h和52 h植物表型,测定受害植株比例,以期为近缘改良植物耐碱性和培育耐碱嵌合体红车轴草提供参考依据。结果表明:野生型能耐受80 mmol·L^(-1)盐胁迫和40 mmol·L^(-1)碱胁迫。将耐碱基因Gshdz4-GFP通过K599发根农杆菌转入红车轴草植株根系,利用激光共聚焦检测绿色荧光蛋白信号,发现Gshdz4基因表达于红车轴草根的外层细胞。该研究测定了未转基因和转基因根系超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量,结果显示转Gshdz4提高植株根系对碱胁迫耐性。Taking seeds of Trifolium pratense as the test material,the methods of treating the seeds and seedlings of Trifolium pratense with 40,80,120,160,and 320 mmol·L^(-1) as well as NaHCO 3 solutions at concentrations of 20,40,60,80,and 160 mmol·L^(-1) were used.Various growth parameters included germination potential and root length were examined under these stress conditions.Plant phenotypes were observed at different time points(0,4,8,24,32,44,48 hours and 52 hours),and the proportion of affected plants was determined,in order to provide insights into improving alkaline tolerance and breeding alkali-resistant chimera Trifolium pratense varieties.The results showed that the wild type could tolerate salt stress up to a concentration of 80 mmol·L^(-1) and alkali stress up to a concentration of 40 mmol·L^(-1).The alkali-tolerant gene GSHDZ4-GFP was introduced into the root system of Trifolium pratense using Agrobacterium K599.The expression pattern of the Gshdz4 gene in Trifolium pratense roots was detected using laser confocal microscopy by observing green fluorescent protein signals.Super oxide dismutase(SOD)activity and malondialdehyde(MDA)contents were measured in both non-transgenic and transgenic roots.The results indicated that transplantation of Gshdz4 increased the tolerance of plant roots to alkali stress.

关 键 词：豆科植物 红车轴草 耐盐碱 Gshdz4 

分 类 号：S541[农业科学—作物学]