锰基纳米平台协同多疗法增强肝癌治疗的研究  

Manganese-based nanoplatforms for enhanced hepatocellular carcinoma therapy

作　　者：闫康宁 刘军杰[1] Yan Kangning;Liu Junjie(Department of Medical Ulrasound,Guangxi Medical University Cancer Hospital,Nanning 530000,China)

机构地区：[1]广西医科大学附属肿瘤医院超声科,南宁530000

出　　处：《现代仪器与医疗》2025年第1期51-57,共7页Modern Instruments & Medical Treatment

基　　金：广西影像医学临床医学研究中心建设(桂科AD20238096)。

摘　　要：目的本研究旨在开发一种负载Mn^(2+)的聚多巴胺纳米平台(Mn-PDA,MD),通过光热疗法联合化学动力疗法,探讨其在肝细胞癌治疗中的机制与效果。方法采用自氧化聚合法合成MD,并通过透射电子显微镜、动态光散射及X射线光电子能谱表征其结构和性质。进一步评估其光热性能、Mn^(2+)的响应释放特性以及类芬顿反应催化活性。在体外实验中,通过CCK-8和活/死染色评估肿瘤细胞存活率,并检测光热效应和Mn^(2+)释放对细胞毒性的影响。结果本研究合成的MD粒径约200nm,经近红外(Near-Infrared,NIR)激光照射7min,温度迅速升至47.2℃。NIR照射触发MD释放Mn^(2+),进一步催化生成羟基自由基。体外实验显示,在100μg/ml和NIR照射(1W/cm^(2),10min)条件下,MD将肿瘤细胞活力降低至40%。活/死细胞染色进一步证实,大量肿瘤细胞发生死亡。与聚多巴胺相比,MD表现出更显著的杀伤能力,差异具有统计学意义。结论本研究开发的MD通过光热效应与Mn^(2+)介导的活性氧的产生,实现了对肿瘤细胞的高效杀伤,具有协同治疗的优势,为肝癌治疗提供了新的策略和方向。Objective This study aims to develop a polydopamine-based nanoplatform loaded with Mn^(2+)(Mn-PDA,MD)and explore its mechanism and efficacy in hepatocellular carcinoma treatment through the combination of photothermal therapy and chemodynamic therapy.Methods MD was synthesized by self-oxidative polymerization and characterized by transmission electron microscopy,dynamic light scattering,and X-ray photoelectron spectroscopy for its structure and properties.Its photothermal performance,Mn^(2+)release characteristics in response to stimuli,and Fenton-like reaction catalytic activity were further evaluated.In vitro experiments were conducted to assess the survival rate of tumor cells using CCK-8 and live/dead staining,and to detect the effects of photothermal effect and Mn^(2+)release on cytotoxicity.Results The synthesized MD had a particle size of approximately 200 nm.After 7 minutes of near-infrared(NIR)laser irradiation(1 W/cm^(2)),the temperature rapidly rose to 47.2℃.NIR irradiation triggered the release of Mn^(2+)from MD,which further catalyzed the generation of hydroxyl radicals.In vitro experiments showed that at a concentration of 100μg/ml and under NIR irradiation(1 W/cm2,10 min),MD reduced the viability of tumor cells to 40%.Live/dead staining further confirmed that a large number of tumor cells died.Compared with polydopamine,MD exhibited more significant killing ability,and the difference was statistically significant.Conclusion The developed MD achieved efficient killing of tumor cells through the combination of photothermal effect and Mn^(2+)-mediated reactive oxygen species generation,demonstrating the advantages of synergistic therapy and providing a new strategy and direction for liver cancer treatment.

关 键 词：纳米材料 光热疗法 化学动力学疗法 锰离子 肝细胞癌 

分 类 号：TH77[机械工程—仪器科学与技术] R445.2[机械工程—精密仪器及机械]