Nanoparticle Induces Membrane Fusion in a State-wise and Property-sensitive Mode  

作　　者：Chi-Yun Ma Xue-Wei Dong Xue-Mei Lu Bing Yuan Kai Yang 

机构地区：[1]Center for Soft Condensed Matter Physics and Interdisciplinary Research&School of Physical Science and Technology,Soochow University,Suzhou,215006,China [2]State Key Laboratory of Surface Physics and Department of Physics,Fudan University,Shanghai,200433,China [3]School of Optical and Electronic Information,Suzhou City University,Suzhou,215104,China [4]School of Physical Science and Technology,Jiangsu Key Laboratory of Thin Films,Soochow University,Suzhou,215006,China [5]Songshan Lake Materials Laboratory,Dongguan,523808,China

出　　处：《Chinese Journal of Polymer Science》2025年第1期235-244,I0014,共11页高分子科学(英文版)

基　　金：supported by the National Natural Science Foundation of China(Nos.22303060,12274307,32230063,12347102 and 22203059);Natural Science Foundation of Jiangsu Province(Nos.BK20230470 and BK20210100);the Natural Science Foundation of Guangdong Province of China(No.2023A1515011610);the Program of Jiangsu Provincial Key Lab of Thin Films(No.KJS2131);the open research fund of State Key Laboratory of Surface Physics of Fudan University(KF2023_03).

摘　　要：Membrane fusion is essential for many cellular physiological functions,which is modulated by highly precise molecular mechanism involving multiple energy barriers.Nanoparticles(NPs),which exhibit immense potential in the field of biomedical applications,can act as fusogen proteins to initiate and regulate membrane fusion.However,the underlying mechanisms of NP-induced membrane fusion and the molecular details involved remain largely elusive.Here,using coarse-grained molecular dynamics simulations,we systematically investigate the NP-induced membrane fusion behaviors and the influences of NP properties(size,hydrophobicity and hydrophilicity).Our results show that the vesicle-bilayer fusion induced by a hydrophobic NP is an intricately state-wise process,involving the approach and local deformation of the vesicle and bilayer bridging by the NP,the flip-flop of lipids from proximal leaflets and the formation of a fusion stalk,as well as further lipid interactions between distal leaflets and complete fusion.Moreover,we find that NP properties have distinct effects on membrane fusion and thus the optimal NP conditions for facilitating membrane fusion are obtained.Our work provides a mechanistic understanding of NP-induced membrane fusion and offers useful insights for efficient and controlled regulation of membrane fusion.

关 键 词：NANOPARTICLE Membrane fusion Molecular dynamics simulation Coarse-grained model 

分 类 号：TB3[一般工业技术—材料科学与工程]