Two FT genes synergistically regulate the reproductive transition of loquat  

作　　者：Yuanyuan Jiang Yunmei Zhu Ze Peng Wenbing Su Jiangrong Peng Yuan Yuan Ling Zhang Zhike Zhang Xianghui Yang Yongshun Gao Shunquan Lin Chongjian Ma 

机构地区：[1]Guangdong Provincial Key Laboratory of Utilization and Conservation of Food and Medicinal Resources in Northern Region,Shaoguan University,Shaoguan,Guangdong,512005,China [2]North Guangdong Engineering Technology Research Center for the Efficient Utilization of Water and Soil Resources,Shaoguan University,Shaoguan,Guangdong,512005,China [3]State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources,College of Horticulture,South China Agricultural University,Guangzhou,Guangdong,510642,China [4]School of Biology and Agriculture,Shaoguan University,Shaoguan,Guangdong,512005,China [5]Nanping Institute of Agricultural Sciences,Jianyang,Fujian,354200,China [6]Fruit Research Institute,Fujian Academy of Agricultural Science,Fuzhou,Fujian,350013,China [7]Institute of Forestry and Pomology,Beijing Academy of Agriculture and Forestry Sciences,Beijing,100093,China

出　　处：《Horticultural Plant Journal》2025年第2期548-563,共16页园艺学报(英文版)

基　　金：supported by the Guangdong Basic and Applied Basic Research Foundation(Grant No.2024A1515013095,2021A1515011048);the Open Fund of the Guangdong Provincial Key Laboratory of Utilization and Conservation of Food and Medicinal Resources in Northern Region(Grant No.SY-KFFMR2022009Z);Shaoguan City Science and Technology Planning Project(Social Development Direction-Supporting Scientific Research Workers Project)(Grant No.200811094530739)。

摘　　要：Unlike annual flowering plants that exhibit a single reproductive cycle encompassing vegetative growth,flowering,and senescence within a single year,perennial woody trees undergo a transition between vegetative and reproductive growth every year after a long juvenile phase.Most woody fruit trees bloom when it turns warm in spring,and the fruit ripens in summer or autumn.However,as a member of the Maloideae subfamily,the loquat tree blooms in the cold autumn and winter,and the fruit matures in the spring.To explore the regulatory mechanism of loquat flowering,we characterized two FLOWERING LOCUS T homologous genes in loquat,EjFT1 and EjFT2.qRT-PCR results revealed that EjFT1 and EjFT2 exhibited nearly opposite expression patterns in various tissues.EjFT1 was mainly expressed in young tissues;EjFT2 was mainly expressed in mature leaves,open flowers,and fruits.EjFT2 exhibited an obvious circadian rhythm and was regulated by EjCO.After the trees were exposed to short-day conditions or sprayed with exogenous GA_(3),the expression of EjFT2 was strongly inhibited,and the loquat tree did not produce floral buds.Furthermore,the yeast two-hybrid,bimolecular fluorescence complementation,and Dualluciferase assay experiments revealed that both EjFT1 and EjFT2 interacted with EjFD,with the EjFT2-EjFD protein complex enhancing the activity of EjAP1-1 and EjAP1-2 promoters,while EjFT1-EjFD inhibited the activity of the EjAP1-1 promoter.Protein structural analysis of EjFT1and EjFT2 suggested that differences in amino acid residues at Val_(123)/Leu_(123),Ser_(157)/Ala_(157),and Val_(158)/Ala_(158)may be the reason for their functional differences.Our results showed that EjFT1 and EjFT2 may cooperatively regulate the floral bud differentiation of loquat by competitively binding with EjFD.EjFT2 regulates the onset of loquat floral bud differentiation by responding to photoperiod and gibberellin signals,while EjFT1 is involved in the vegetative growth of loquat.These findings provide important clues for the investigation of the regul

关 键 词：LOQUAT Flowering time GA3 Short day FT AP1 

分 类 号：S667.3[农业科学—果树学]