Genome-wide analysis of the S-phase kinase-association protein1(ClSKP1) family and the role of S-RNase targeting by an SCF(Cullin1-SKP1-F-box) complex in the self-incompatibility of‘Xiangshui' lemon  

作　　者：Yuze Li Wei Lin Jiawei Zhu Moying Lan Cong Luo Yili Zhang Rongzhen Liang Liming Xia Wangli Hu Xiao Mo Guixiang Huang Xinhua He 

机构地区：[1]State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources,College of Agriculture,Guangxi University,Nanning,Guangxi 530004,China [2]Key Laboratory of Bio-resource and Eco-environment of Ministry of Education,College of Life Sciences,Sichuan University,Chengdu,Sichuan 610065,China [3]Institute of Subtropical Agriculture,Fujian Academy of Agricultural Sciences,Zhangzhou,Fujian 363005,China

出　　处：《Horticultural Plant Journal》2025年第2期593-607,共15页园艺学报(英文版)

基　　金：supported by grants from the National Natural Science Foundation of China(Grant No.31960585);Science and Technology Major Project of Guangxi(Grant No.Guike AA22068092);Guangxi Science and Technology Vanguard Special Action Project(Grant No.202204);State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources(Grant Nos.SKLCUSA-a201906,SKLCU-SA-c201901)。

摘　　要：The SKP1 gene is an important component of the SCF(SKP1-Cullin1-F-box)complex and serves as a bridge connecting the F-box and Cullin1genes(F-box-SKP1-Cullin1).The pattern of S-RNase being ubiquitously labelled by the SCF complex and degraded by the 26S protease accounts for the bulk of the available self-incompatibility studies.In this study,15 ClSKP1s from the‘Xiangshui'lemon genome and ubiquitome exist in the same SKP1 conserved domain(CD)as SKP1s in other species.The q PCR results showed that SKP1-6 and SKP1-14 have tissue expression patterns specific for expression in pollen.In addition,SKP1-6 and SKP1-14 in the stigma,style and ovary were significantly upregulated after self-pollination compared to those after cross-pollination.A subcellular location showed that SKP1-6 and SKP1-14 were located in the nucleus.In addition,yeast two-hybrid(Y2H)assays,bimolecular fluorescence complementation(BiFC)and luciferase complementation imaging(LCI)assays showed that SKP1-6 interacted with F-box1,F-box33,F-box34,F-box17,F-box19,Cullin1-2 and 26S proteasome subunit 4 homolog A(26S PS4HA).SKP1-14 interacted with F-box17,F-box19,F-box35,Cullin1-2 and 26S PS4HA.The interaction of Cullin1-2 and the F-box with SKP1 as a bridge was verified by a yeast three-hybrid experiment.The ability of S3-RNase to inhibit pollen and pollen tube growth and development was assessed using in vitro pollen co-culture experiments with recombinant S3-RNase proteins.Overall,this study provides important experimental evidence and theoretical basis for understanding the mechanism of self-incompatibility in plants by revealing the key role of the SCF complex in‘Xiangshui'lemon,which is bridged by ClSKP1-6,in self-incompatibility.The results of this study are of great significance for the future indepth exploration of the molecular mechanism of the SCF complex and its wide application in the self-incompatibility of plants.

关 键 词：LEMON SKP1 SCF SELF-INCOMPATIBILITY Expression analysis Functional analysis 

分 类 号：S666.5[农业科学—果树学]