Comparative transcriptomics analysis reveals stage-specific gene expression profiles associated with gamete formation in Garlic  

作　　者：Jie Ge Jide Fan Yongqiang Zhao Xinjuan Lu Canyu Liu Biwei Zhang Qingqing Yang Mengqian Li Yan Yang Feng Yang 

机构地区：[1]Xuzhou Institute of Agricultural Sciences in Jiangsu Xuhuai Area,Key Laboratory of Biology and Genetic Breeding of Sweetpotato,Ministry of Agriculture and Rural Affairs,Xuzhou,Jiangsu 221131,China

出　　处：《Horticultural Plant Journal》2025年第2期839-853,共15页园艺学报(英文版)

基　　金：supported by the National Characteristic Vegetable Industry Technology System of China(Grant No.CARS24-A-07);the Jiangsu Modern Agricultural Industry Technology System Construction Special Fund(Grant No.JATS[2023]050);Xuzhou Academy of Agricultural Sciences Research Fund Project(Grant No.XM2021003)。

摘　　要：Commercial cultivars of garlic,a popular condiment,are sterile,making genetic variation and germplasm innovation of this plant challenging.Understanding mechanism of gamete sterility in garlic and their key regulatory networks is therefore important for fertility restoration.In this work,we conducted a detailed phenotypic analysis of fertile and sterile garlic genotypes and found that enlargement of topset in the inflorescence of sterile genotypes led to abnormal flowers.Additional cytological observations showed that aberrant meiotic cytokinesis in sterile garlic ultimately resulted in pollen degeneration.Transcriptomics analysis of sterile and fertile genotypes identified possible molecular mechanisms underlying gamete abortion.A total of 100710 differentially expressed genes(DEGs)between the fertile and sterile garlic flowers at three stages of gamete development were identified,many of which were involved in homologous chromosome synapsis during meiosis,MYB transcription factor regulation,ribosome biogenesis and plant hormone signal transduction.Taken together,these results provide insight into the molecular mechanisms and regulatory networks underlying gamete development in garlic and point to a set of candidate genes for further functional characterization.

关 键 词：Allium sativum L. STERILITY MEIOSIS Genome expression Transcription factor TRANSCRIPTOME Ribosome biogenesis 

分 类 号：S633.4[农业科学—蔬菜学]