Ca^(2+)-induced CpCBL8-CpCIPK9 module negatively regulates dormancy breaking and cold tolerance in winter-flowering wintersweet  

作　　者：Mingkang Tian Qiang Li Ning Liu Juncai Li Juntao Huo Shunzhao Sui Zhineng Li 

机构地区：[1]Key Laboratory of Agricultural Biosafety and Green Production of Upper Yangtze River(Ministry of Education),Chongqing Engineering Research Center for Floriculture,College of Horticulture and Landscape Architecture,Southwest University,Chongqing,400715,China [2]Integrative Science Center of Germplasm Creation in Western China(CHONGQING)Science City,Southwest University,Chongqing 400712,China

出　　处：《Horticultural Plant Journal》2025年第2期877-890,共14页园艺学报(英文版)

基　　金：funded by the Natural Science Foundation of Chongqing(CSTB2023NSCQ-MSX0236);Fundamental Research Funds for the Central Universities(SWU-XDJH202308);Earmarked Funds for the China Agriculture Research System(CARS-26)。

摘　　要：The complex of calcineurin B-like protein(CBL)and CBL-interacting protein kinase(CIPK)serves as key components in calcium-sensing,orchestrating various signals crucial for plant growth,development,and responses to biotic and abiotic stresses.However,the mechanism underlying the response of this module to cold stress and its role in flower development in wintersweet(Chimonanthus praecox)remains unclear.Through expression pattern analysis,calcium ion(Ca^(2+))concentration assays,correlation analysis,and linear regression analysis,we found that the[Ca^(2+)],along with CpCBL8 and CpCIPK9 expression levels in wintersweet flower buds(FBs),significantly decreased during the initial flowering stage when the chilling requirement reached 570 chill units(CU).Notably,there was a significant positive correlation between[Ca^(2+)]and CpCBL8 expression.Ca^(2+)increased the expression of Cp CBL8 and CpCIPK9 in FBs,causing a significant delay in the flowering of wintersweet.Furthermore,the function of CpCBL8 was studied using heterologous transformation.Overexpression of CpCBL8 significantly delayed flowering time and significantly reduced cold tolerance and altered the expression pattern of endogenous genes related to low-temperature stress and flower development in transgenic Arabidopsis thaliana.Additionally,transcriptome analysis of chilling-induced dormancy breaking and flower bud enlargement revealed that CpCBL8 and CpCIPK9 were negatively regulated by cold,and the expression pattern of endogenous genes related to flower development and cold stress in wintersweet were similar to that of in A.thaliana.Moreover,protein-protein interaction(PPI)analysis revealed that CpCBL8 and CpCIPK9 interacted in the plasma membrane and nucleus.On the basis of these findings,we speculated that the CpCBL8-CpCIPK9 module plays a crucial role in regulating responses to cold stress and flower development in wintersweet.This study elucidated molecular mechanisms through which the downregulation of the Ca^(2+)-induced CpCBL8-CpCIPK9 module results

关 键 词：Chimonanthus praecox CA^(2+) Calcineurin B-like protein(CBL) CBL-interacting protein kinase(CIPK) Flower development Cold stress response 

分 类 号：S685.99[农业科学—观赏园艺]