A molecular module with Phe IAA17 as the core significantly promotes lateral root germination  

作　　者：Junlei Xu Miaomiao Cai Yali Xiea Zhanchao Cheng Chongyang Wu Jian Gao 

机构地区：[1]Key Laboratory of National Forestry and Grassland Administration/Beijing for Bamboo&Rattan Science and Technology,International Centre for Bamboo and Rattan,Beijing 100102,China [2]Department of Biology and Food Engineering,Bozhou University,Bozhou,Anhui 236800,China

出　　处：《Horticultural Plant Journal》2025年第2期921-934,共14页园艺学报(英文版)

基　　金：supported by the National Key Research and Development Program of China(2021YFD2200505);the National Natural Science Foundation of China(32071849)。

摘　　要：Monocot root systems comprise a large number of lateral roots to allow them to survive and colonize land.Auxin signaling pathways centered on Aux/IAA play a crucial role in lateral root development.However,in non-model monocot plants,the effects of Aux/IAA on lateral root initiation and number remain largely unknown.The present study transformed PheIAA17,a member of the Aux/IAA family of Moso bamboo,into rice and found that it significantly drove plants to produce lateral roots and improved the rooting rate.Quantitative experiments showed that PheIAA17 overexpression significantly affected the expression of ARF family members.Phylogenetic and promoter analyses indicate that PheARF3-2 belongs to class B ARF,and the promoter region contains auxin response elements.The results of yeast one-hybrid and dual-luciferase reporter assays confirmed that PheIAA17 bound specific fragments of the PheARF3-2 promoter to repress its transcriptional activity.Y2H and BiFC assay have shown that PheIAA17 and PheIAA30-3 could physically interact in vitro and in vivo.Taken together,this study reports a new molecular module centered on PheIAA17,which directs plants to alter root morphology through an increase in lateral roots.

关 键 词：Phyllostachys edulis Moso bamboo Lateral root PheIAA17 Auxin signaling Molecular module Class B ARF 

分 类 号：Q945[生物学—植物学]