龟鹿桃红方对精索静脉曲张模型大鼠精液质量及生精细胞凋亡的影响  

Effect of Guilu Taohong Formula on semen quality and spermatogenic cell apoptosis in a varicocele model of rats

作　　者：王彪 杨扬 邱泽锐 冯恩敏 赵祥 王能 黄鑫 林群芳[1] 周青[1] WANG Biao;YANG Yang;QIU Ze-rui;FENG En-min;ZHAO Xiang;WANG Neng;HUANG Xin;LIN Qun-fang;ZHOU Qing(First Department of Male Surgical Miscellaneous Diseases,The First Affiliated Hospital of Hunan University of Chinese Medicine,Changsha,Hunan 410007,China)

机构地区：[1]湖南中医药大学第一附属医院男性病外科杂病一科,湖南长沙410007

出　　处：《中华男科学杂志》2025年第2期150-156,共7页National Journal of Andrology

基　　金：湖南省教育厅科学研究(优秀青年)项目(22B0368);国家中医优势专科外科(国中医药医政函〔2024〕90号);湖南省一类中医重点专科(中医男科)[湘中医药函(2023)4号]。

摘　　要：目的:观察龟鹿桃红方对精索静脉曲张(VC)模型大鼠精液质量的影响并探究其可能机制。方法:48只SD雄性大鼠随机分为假手术组、模型组、龟鹿桃红方组、左卡尼汀组,模型建立后连续灌胃给药8周。观察大鼠一般情况,灌胃结束后计算睾丸、附睾指数,全自动精液分析仪检测大鼠精液质量,TUNEL染色观察睾丸组织细胞凋亡情况,Western印迹检测睾丸组织B淋巴细胞瘤-2(Bcl-2)、Bcl-2相关X蛋白(Bax)、半胱氨酸天冬氨酸蛋白酶-3(caspase-3)的表达水平。结果:与假手术组相比,模型组睾丸指数、附睾指数、精子浓度、前向运动精子百分率均显著下降(P<0.01),生精细胞凋亡率及Bax、caspase-3蛋白表达量均显著上升(P<0.01),Bcl-2蛋白表达量显著下降(P<0.01)。与模型组相比,龟鹿桃红方组睾丸指数、附睾指数及精子浓度、前向运动精子百分率均显著上升(P<0.05或P<0.01),生精细胞凋亡率及Bax、caspase-3蛋白表达量均显著下降(P<0.01),Bcl-2蛋白表达量显著上升(P<0.01)。与模型组相比,左卡尼汀组睾丸指数、附睾指数及精子浓度、前向运动精子百分率均显著上升(P<0.05或P<0.01),生精细胞凋亡率及Bax、caspase-3蛋白表达量均显著下降(P<0.01或P<0.05),Bcl-2蛋白表达量显著上升(P<0.01)。结论:龟鹿桃红方能改善VC模型大鼠精液质量,降低睾丸组织生精细胞凋亡率,可能与促进Bcl-2蛋白表达,抑制Bax、caspase-3蛋白表达水平有关。Objective:To observe the effect of Guilu Taohong Formula on semen quality in varicocele(VC)models of rats,and to explore its possible mechanism.Methods:Forty-eight male SD rats were randomly divided into four groups(sham group,model group,Guilu Taohong Formula group and L-carnitine group).After the establishment of models,the rats were treated with intragastric administration for eight consecutive weeks.The general condition of the rats was observed.After the gavage,the testicular and epididymal indices were calculated.Semen quality was assessed using an automatic semen analyzer.Apoptosis of testicular cells was assessed by TUNEL staining.And the expression levels of B-cell lymphocytoma-2(Bcl-2),Bcl-2-associated X protein(Bax)and cysteine aspartate protease-3(caspase-3)in testicular tissue were detected by Western blot.Results:Compared with the sham group,testicular index,epididymal index,sperm concentration,the percentage of progressive motility of sperm(PR%)and the expression level of Bcl-2 decreased in model group(P<0.01).An increased apoptosis rate of spermatogenic cells and the expression levels of Bax and caspase-3 proteins were observed in model group as well(P<0.01).Compared with the model group,the testicular index,epididymal index,sperm concentration,PR%and the expression level of Bcl-2 in Guilu Taohong Formula group increased significantly(P<0.05,P<0.01).A decreased apoptosis rate of spermatogenic cells and the expression levels of Bax and caspase-3 proteins were detected in Guilu Taohong Formula group as well(P<0.01).Similarly,the L-carnitine group showed increased testicular index,epididymal index,sperm concentration,PR%and the expression level of Bcl-2 protein(P<0.05,P<0.01),where showed decreased apoptosis rate of spermatogenic cells and the expression levels of Bax and caspase-3 proteins compared with model group(P<0.01,P<0.05).Conclusion:Guilu Taohong Formula improves semen quality in VC model rats and reduces the apoptosis rate of spermatogenic cells in testicular tissue,which may be related to

关 键 词：精索静脉曲张 龟鹿桃红方 细胞凋亡 精液质量 肾虚血瘀 大鼠 

分 类 号：R697.24[医药卫生—泌尿科学]