Photoactivated SOPP3 enables APEX2-mediated proximity labeling with high spatio-temporal resolution in live cells  

作　　者：Dajun Qu Yaxin Li Qian Liu Biao Cao Mengye Cao Xiaoxi Lin Chengxing Shen Peng Zou Hu Zhou Wenjuan Zhang Weijun Pan 

机构地区：[1]State Key Laboratory of Experimental Hematology,Haihe Laboratory of Cell Ecosystem,Shanghai Institute of Nutrition and Health,University of Chinese Academy of Sciences,Chinese Academy of Sciences,Shanghai,China [2]Department of Analytical Chemistry,State Key Laboratory of Drug Research,Shanghai Institute of Materia Medica,Chinese Academy of Sciences,Shanghai,China [3]School of Medicine,Shanghai Jiao Tong University,Shanghai,China [4]College of Chemistry and Molecular Engineering,Synthetic and Functional Biomolecules Center,Beijing National Laboratory for Molecular Sciences,Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education,Peking University,Beijing,China

出　　处：《Cell Research》2025年第2期149-152,共4页细胞研究(英文版)

基　　金：supported by the National Key R&D Program of China(2023YFA1802000,2018YFA0800203);the National Natural Science Foundation of China for Distinguished Young Scholars(31925014);the National Natural Science Foundation of China Key Program(32130033);the National Natural Science Foundation of Original Exploratory Program(32350006);the Key Research Program of Frontier Sciences,Chinese Academy of Sciences(ZDBS-LYSM010);Shanghai Pilot Program for Basic Research-Chinese Academy of Sciences,Shanghai Branch(JCYJ-SHFY-2022-006);Shanghai Science Technology Innovation Action Plan for Basic Research Program(21JC1406300);Haihe Laboratory of Cell Ecosystem Innovation Fund(24HHXBSS00011);CAS project for Young Scientists in Basic Research(YSBR-077);W.P.,and the National Key R&D Program of China(2022YFA1302902)to H.Z.

摘　　要：Dear Editor,Proximity labeling(PL)is an emerging technology for probing protein interactome in living cells.Current PL technology,like using engineered peroxidases(APEX2,HRP),is widely employed,benefiting from their high temporal resolution.^(1,2)However,cytotoxicity of exogenous H_(2)O_(2),which is used to activate APEX2,largely limits its application.Meanwhile,PL methods employing biotin ligases,such as BioID,^(3)BioID2,^(4)TurboID,5 give poor temporal resolution due to low catalytic activity and prolonged labeling time.An approach that could catalyze PL in living cells with high spatio-temporal resolution is still missing.

关 键 词：RESOLUTION enable PROXIMITY 

分 类 号：O64[理学—物理化学]