高温胁迫对抽穗扬花期水稻丙二醛含量和抗氧化酶活性的影响  

Effects of High Temperature Stress on the MDA Content and Antioxidant Enzyme Activity of Rice at Heading and Flowering Stage

作　　者：黄乾龙[1] 李贤勇[1] 欧阳杰[1] 朱子超[1] 何永歆[1] 蒋刚[1] 管玉圣[1] 熊英[1] 王楚桃[1] HUANG Qianlong;LI Xianyong;OUYANG Jie;ZHU Zichao;HE Yongxin;JIANG Gang;GUAN Yusheng;XIONG Ying;WANG Chutao(Chongqing Academy of Agricultural Sciences,Chongqing 401329,China)

机构地区：[1]重庆市农业科学院,重庆401329

出　　处：《杂交水稻》2025年第1期21-26,共6页Hybrid Rice

基　　金：重庆市科研机构绩效激励引导专项(cstc2022jxjl80026);重庆市技术创新与应用发展专项(CSTB2022TIADKPX0018)。

摘　　要：为探明水稻的耐热生理基础,以自育水稻亲本神9B、Q恢28、Q3B、Q恢18为材料,对不同材料的耐热水平进行分析,并探究高温胁迫对抽穗扬花期供试材料主茎剑叶丙二醛(MDA)含量和抗氧化酶活性的影响。结果表明,耐热型材料(神9B和Q恢28)的结实率降幅显著低于不耐热型材料(Q3B和Q恢18)。与适温对照相比,高温胁迫下各供试材料MDA含量均显著增加,不耐热型材料的增幅显著高于耐热型材料;抗氧化酶[包括超氧化物歧化酶(SOD)、过氧化物歧化酶(POD)、过氧化氢酶(CAT)]活性均显著增加,且耐热型材料的POD、CAT活性增幅显著高于不耐热型材料。供试材料的结实率降幅与POD活性呈显著负相关。综上,结实率、MDA含量、POD和CAT活性可作为衡量抽穗扬花期水稻耐热性的良好指标。In order to explore the physiological basis of heat tolerance of rice,the heat tolerance levels of self-bred rice parents Shen 9B,Q hui 28,Q3B and Q hui 18 were analyzed,and the effects of high temperature stress on the MDA content and the activity of antioxidant enzymes in the flag leaf of the tested materials at heading and flowering stage were studied.The results showed that the decreasing rate of seed setting rate of the heat-tolerant materials(Shen 9B and Q hui 28)was significantly lower than that of the heat-sensitive materials(Q3B and Q hui 18).Compared with the appropriate temperature control,the MDA content of the tested materials increased significantly under high temperature stress and the increasing rate of the heat-sensitive materials was significantly higher than that of the heat-tolerant materials;the activities of antioxidant enzymes(including superoxide dismutase(SOD),peroxide dismutase(POD)and catalase(CAT))increased significantly under high temperature stress,and the increasing rate of POD and CAT activities in the heat-tolerant materials was significantly higher than that in the heat-sensitive materials.There was a significant negative correlation between the decreasing rate of seed setting rate and the POD activity.In conclusion,the seed setting rate,MDA content,POD and CAT activity can be used as good indicators to evaluate the heat tolerance of rice at heading and flowering stage.

关 键 词：高温胁迫 MDA含量 抗氧化酶活性 抽穗扬花期 水稻 

分 类 号：S511.01[农业科学—作物学] S311