桑树桑黄总三萜的提取纯化及其生物活性研究  

Extraction,purification and biological activity of total triterpenes from Sanghuangporus sanghuang

作　　者：刘坤[1,2] 付鑫垚 郭彤 张婉月 赵庆生 孔泽娟[4] 程华 宋恒[5] LIU Kun;FU Xinyao;GUO Tong;ZHANG Wanyue;ZHAO Qingsheng;KONG Zejuan;CHENG Hua;SONG Heng(College of Bioscience and Bioengineering,Hebei University of Economics and Business,Shijiazhuang 050061,China;Department of Mathematics and Statistics,Hebei University of Economics and Business,Shijiazhuang 050061,China;Institute of Process Engineering,Chinese Academy of Sciences,Beijing 100190,China;Institute of Biology,Hebei Academy of Sciences,Shijiazhuang 050081,China;Research Office of Sanghuangporus sanghuang,Peace Angel(Zhejiang)Traditional Chinese Medicine Slices Co.,Ltd.,Jiaxing 314000,Zhejiang,China)

机构地区：[1]河北经贸大学生物科学与工程学院,石家庄050061 [2]河北经贸大学数学与统计学院,石家庄050061 [3]中国科学院过程工程研究所,北京100190 [4]河北省科学院生物研究所,石家庄050081 [5]太安谷(浙江)中药饮片有限公司桑树桑黄研究室,浙江嘉兴314000

出　　处：《广西植物》2025年第2期217-227,共11页Guihaia

基　　金：河北省重点研发计划项目(21327109D);河北省科学院基本科研业务费制度试点项目(2024PF04)。

摘　　要：为了优化桑树桑黄总三萜的提取工艺,纯化总三萜并分析其化学成分及其抗肿瘤和抗氧化活性。该研究采用响应面法优化桑树桑黄总三萜的提取工艺,经D101型大孔树脂和Sephadex LH-20层析联用纯化总三萜,结合UHPLC-ESI-MS技术分析三萜成分,并通过CCK8法、DPPH法和ABTS法对其进行抗肿瘤和抗氧化活性测定。结果表明:(1)最佳提取工艺为提取时间55 min、提取温度45℃、乙醇浓度75%、液料比35∶1(mL·g^(-1)),此条件下总三萜含量为10.34 mg·g^(-1)。(2)纯化后的Fraction a(Fa)组分纯度为47.98%,提高了3.8倍;从Fa中检测出8种三萜成分,即甘草次酸、黄柏酮、熊果酸、大戟醇、24,25-二氢羊毛甾醇、大豆皂苷B、羊毛甾醇、山楂酸。(3)200μg·mL^(-1)时Fa对肿瘤细胞PC3的抑制率为68.65%,高于5-氟尿嘧啶66.30%;Fa对DPPH自由基和ABTS自由基均有较高的清除能力,半数清除率分别为42.76μg·mL^(-1)和66.24μg·mL^(-1)。该研究为桑树桑黄总三萜的进一步研究和利用提供理论基础。In order to optimize the extraction process of total triterpenes from Sanghuangporus sanghuang,to purify total triterpenes,and to analyze its chemical components,as well as its antitumor and antioxidant activities.The response surface method was used to optimize the extraction process of total triterpenes from S.sanghuang.D101 macroporous resin and Sephadex LH-20 chromatography were used to purify total triterpenes.Meanwhile UHPLC-ESI-MS technology was used to analyze the component of triterpenes.The antitumor and antioxidant activities of total triterpenes were studied by the methods of CCK8,DPPH and ABTS.The results were as follows:(1)The optimal conditions were extraction time 55 min,extraction temperature 45℃,ethanol concentration 75%and liquid-solid ratio of 35∶1(mL·g^(-1)).Under these conditions,the total triterpenes content was 10.34 mg·g^(-1).(2)The purity of Fraction a(Fa)was increased by 3.8 times to 47.98%.Eight triterpene components were identified from Fa,those were glycyrrhetinic acid,obakunone,ursolic acid,euphol,24,25-dihydrolanosterol,soyasaponin B,lanosterol and maslinic acid.(3)The inhibition rate of Fa on PC3 was 68.65%at 200μg·mL^(-1),which was higher than that of 5-fluorouracil(66.30%).Fa exhibited high DPPH and ABTS free radical scavenging capacities,and scavenging rates with IC_(50) values of 42.76μg·mL^(-1) and 66.24μg·mL^(-1),respectively.This study provides a theoretical basis for further research and utilization of total triterpenes from S.sanghuang.

关 键 词：桑树桑黄 三萜 响应面优化 纯化 抗肿瘤 抗氧化 

分 类 号：Q946[生物学—植物学]