东京银背藤中一个新的异戊烯基异黄酮  

A new prenylated isoflavone from Argyreia pierreana

作　　者：滕明雪 廖广凤 黎云清 钟金明 黄承鸿 刘嘉艺 卢汝梅 TENG Mingxue;LIAO Guangfeng;LI Yunqing;ZHONG Jinming;HUANG Chenghong;LIU Jiayi;LU Rumei(College of Pharmacy,Guangxi University of Chinese Medicine,Nanning 530200,China)

机构地区：[1]广西中医药大学药学院,南宁530200

出　　处：《广西植物》2025年第2期251-261,共11页Guihaia

基　　金：广西科技基地和人才专项(桂科AD20159007);广西中医药大学桂派中医药传承创新团队资助项目(2022B005);2022年自治区中药学研究生联合培养基地开放项目(桂学位[2021]6号);2022年大学生创新创业训练计划项目(S202210600051);2021年大学生创新创业训练计划项目(S202110600113)。

摘　　要：为探究东京银背藤(Argyreia pierreana)的化学成分及其细胞毒活性,该研究采用硅胶、Sephadex LH-20、C18中低压等柱色谱和半制备高效液相色谱等色谱分离手段进行分离纯化,通过理化性质、波谱数据结合参考文献鉴定化合物结构,并采用MTS法测定单体化合物对肿瘤细胞增殖的抑制活性。结果表明:(1)分离得到12个化合物,分别鉴定为argypierin A(1)、4′-羟基-5,7-二甲氧基-6-(3-甲基-丁烯基)-异黄酮(2)、2-羟基-3-甲基蒽醌(3)、rubiadin(4)、rubiadin-1-methyl ether(5)、克罗酰胺(6)、methyl 1,4-di-O-caffeoylquinate(7)、3,4-二-O-咖啡酰基奎宁酸甲酯(8)、3,5-二-O-咖啡酰基奎宁酸甲酯(9)、3,5-二-O-咖啡酰基奎宁酸乙酯(10)、ω-hydroxypropioguaiacone(11)、C-藜芦酰乙二醇(12)。其中,化合物1为新化合物,化合物2-7、10-12为首次从该植物中分离得到,化合物2-7、11、12为首次从银背藤属植物中分离得到。(2)细胞毒活性评价结果显示,化合物6对白血病HL-60、乳腺癌MDA-MB-231、结肠癌SW480肿瘤细胞株具有较好的增殖抑制活性,其IC50值分别为(10.89±0.37)、(16.37±1.13)、(17.35±0.44)μmol·L^(-1)。To study the chemical constituents from Argyreia pierreana and their cytotoxic activities,the compounds were isolated and purified using methods including silica gel,Sephadex LH-20,C 18 medium and low pressure column chromatography and semi-preparative HPLC.The structures of the compounds were identified through a combination method of physicochemical properties,spectral data and compared with literature references.The isolated compounds were tested for their proliferation inhibition effects on tumour cells using MTS method.The results were as follows:(1)Twelve compounds were isolated from the A.pierreana and identified as argypierin A(1),4′-hydroxy-5,7-dimethoxy-6-(3-methyl-butenyl)-isoflavone(2),2-hydroxy-3-methyl anthraquinone(3),rubiadin(4),rubiadin-1-methyl ether(5),grossamide(6),methyl 1,4-di-O-caffeoylquinate(7),3,4-di-O-caffeoyl quinic acid methyl ester(8),3,5-di-O-caffeoyl quinic acid methyl ester(9),3,5-di-O-caffeoyl quinic ethyl ester(10),ω-hydroxypropioguaiacone(11),C-veratrylglycol(12).Among them,Compound 1 was a new compound.Compounds 2-7,10-12 were isolated from A.pierreana for the first time.Compounds 2-7,11,12 were isolated from the genus of Argyreia for the first time.(2)The results of cytotoxic activity evaluation showed Compound 6 had good cytotoxic activities against leukemia HL-60,breast cancer MDA-MB-231 and colon cancer SW480 tumor cell lines with IC 50 values of(10.89±0.37),(16.37±1.13),(17.35±0.44)μmol·L^(-1),respectively.

关 键 词：银背藤属 东京银背藤 异戊烯基异黄酮 克罗酰胺 细胞毒活性 

分 类 号：Q946[生物学—植物学]