壮药水龙的苯丙素和黄酮类成分及其细胞毒活性研究  

Phenylpropanoids and flavonoids from Ludwigia adscendens and their cytotoxicity

作　　者：李金玲 廖广凤 玉其方 谢达晶 陈连 张进燕 卢汝梅 LI Jinling;LIAO Guangfeng;YU Qifang;XIE Dajing;CHEN Lian;ZHANG Jinyan;LU Rumei(College of Pharmacy,Guangxi University of Chinese Medicine,Nanning 530200,China)

机构地区：[1]广西中医药大学药学院,南宁530200

出　　处：《广西植物》2025年第2期296-305,共10页Guihaia

基　　金：广西自然科学基金面上项目(2020GXNSFAA297172);广西壮族自治区级大学生创新创业训练计划立项项目(S202210600045);广西中医药大学桂派中医药传承创新团队资助项目(2022B005)。

摘　　要：为探究壮药水龙(Ludwigia adscendens)的化学成分及其细胞毒活性,该研究采用硅胶、SephadexLH-20、C18中低压和半制备高效液相色谱方法对水龙的化学成分进行分离,通过理化性质以及MS、NMR等波谱方法鉴定化合物结构,并运用MTS法检测单体化合物对5种肿瘤细胞增殖的抑制活性。结果表明:(1)从水龙中分离鉴定15个化合物,包括9个苯丙素类化合物和6个黄酮类化合物,分别为九里香内酯(1)、sibirinol(2)、飞龙掌血酮内酯(3)、飞龙掌血内酯(4)、galgravin(5)、saurufurin C(6)、风藤奎醇(7)、山蒟素C(8)、denudatin B(9)、杨梅苷(10)、desmanthine-2(11)、山奈酚(12)、苜蓿素(13)、杨梅素(14)、3,4′-dimethoxy-5,7,3′-trihydroxyflavone(15),化合物1-9、11、13-15为首次从丁香蓼属植物中分离得到。(2)化合物6对人白血病HL-60、非小细胞肺癌A549、肝癌SMMC-7721、乳腺癌MDA-MB-231和结肠癌SW480有抑制作用,IC50值分别为14.40、27.35、12.19、31.67、33.53μmol·L^(-1);化合物11、12、15对人肝癌SMMC-7721有抑制作用,IC50值分别为31.61、30.71、9.17μmol·L^(-1)。该研究结果丰富了丁香蓼属植物的化学成分,为深入开展水龙抗肿瘤活性研究提供了参考。To study the chemical constituents from Ludwigia adscendens and their cytotoxic activities.Silica gel,Sephadex LH-20,Flash C 18 and semi-preparative liquid chromatography were used to separate the chemical constituents of L.adscendens.Their structures were identified by physicochemical properties and spectral methods such as MS and NMR,and the inhibitory activity of monomeric compounds on the proliferation of five types of tumor cells were detected by MTS method.The results were as follows:(1)A total of 15 compounds were isolated from L.adscendens and identified as coumurrayin(1),sibirinol(2),toddanone(3),toddalolactone(4),galgravin(5),saurufurin C(6),futoquinol(7),hancinone C(8),denudatin B(9),myricitrin(10),desmanthine-2(11),kaempferol(12),tricin(13),myricetin(14),and 3,4′-dimethoxy-5,7,3′-trihydroxyflavone(15).Compounds 1-9,11,and 13-15 were isolated from Ludwigia for the first time.(2)Compound 6 showed significant inhibitory activity against human leukemia HL-60,non-small cell lung cancer A549,liver cancer SMMC-7721,breast cancer MDA-MB-231,and colon cancer SW480,with the IC 50 values of 14.40,27.35,12.19,31.67,and 33.53μmol·L^(-1),respectively.Compounds 11,12,15 showed inhibitory effects on human liver cancer SMMC-7721 with the IC 50 values of 31.61,30.71,and 9.17μmol·L^(-1),respectively.This study enriches the chemical constituents of Ludwigia and provides the reference for further research on the antitumor activity of L.adscendens.

关 键 词：水龙 丁香蓼属 苯丙素类 黄酮类 抗肿瘤活性 

分 类 号：Q946[生物学—植物学]