鬼点灯化学成分及抗结直肠癌活性研究  

Chemical constituents and anti-colorectal cancer activity of Bothriospermum zeylanicum

作　　者：赵小超 柴玲[2] 林霄 商勋 葛竹妹 万广闻 ZHAO Xiaochao;CHAI Ling;LIN Xiao;SHANG Xun;GE Zhumei;WAN Guangwen(Suqian Affiliated Hospital of Nanjing University of Chinese Medicine,Suqian 223800,Jiangsu,China;Guangxi Key Laboratory of Traditional Chinese Medicine Quality Standards,Nanning 530000,China;Institute of Marine Medicine,Guangxi University of Traditional Chinese Medicine,Nanning 530000,China)

机构地区：[1]南京中医药大学宿迁附属医院,江苏宿迁223800 [2]广西中医药研究院广西中药质量标准研究重点实验室,南宁530000 [3]广西中医药大学海洋药物研究院,南宁530000

出　　处：《广西植物》2025年第2期328-336,共9页Guihaia

基　　金：2022年度“宿迁英才”群英计划青年项目;宿迁市科技局自然科学基金青年科技人才项目(K202202);金华市科技局青年人才基金项目(2023KR03);广西科技基地和人才专项(桂科AD17195002)。

摘　　要：为探究鬼点灯(Bothriospermum zeylanicum)的抗结直肠癌活性成分,该研究采用ODS反相柱色谱、高压制备液相色谱等方法对乙酸乙酯部进行分离纯化,通过质谱及核磁共振等波谱分析鉴定化合物结构,并采用CCK8法检测化合物对人结直肠癌细胞(SW620、HT-29)及人正常结肠上皮细胞(NCM460)增殖的影响。结果表明:(1)从鬼点灯乙酸乙酯部共分离得到12个化合物,分别鉴定为富马酸(1)、原儿茶酸(2)、富马酸单甲酯(3)、3,4,α-三羟基甲基苯丙酸酯(4)、对羟基苯甲酸(5)、echiumin A(6)、echiumin B(7)、(+)-异落叶松脂素-2a-O-β-D-葡萄糖苷(8)、对香豆酰-α-L-鼠李吡喃糖苷(9)、对香豆酸(10)、(-)-5′-甲氧基异落叶松脂醇-3α-O-β-D-葡萄糖苷(11)、rupestrin B(12)。化合物1-12均为首次从鬼点灯中分离得到。(2)化合物8和化合物12对SW620细胞增殖具有一定抑制作用,其IC50值分别为(9.34±1.10)μmol·L^(-1)和(0.33±0.06)μmol·L^(-1),化合物12对HT-29细胞增殖具有一定的抑制作用,其IC50值为(15.94±1.56)μmol·L^(-1)。该研究结果丰富了鬼点灯的化学成分,进一步明确了其抗结直肠癌活性成分,为鬼点灯的药效开发与利用提供了参考。In order to explore the anti-colorectal cancer activity constituents of Bothriospermum zeylanicum,the compounds were isolated from the ethyl acetate fraction by ODS reversed phase column chromatography,high-pressure preparative liquid chromatography and other methods.MS,NMR and other spectroscopic were used to identify the structure of the compounds.And then the effects of the compounds on the proliferation of human colorectal cancer cells(SW620,HT-29)and human normal colon epithelial cells(NCM460)were detected by CCK8 method.The results were as follows:(1)A total of 12 compounds were identified from the ethyl acetate fraction of B.zeylanicum,including fumaric acid(1),protocatechuic acid(2),monomethyl fumarate(3),3,4,α-trihydroxymethylphenyl propionate(4),p-hydroxybenzoic acid(5),echiumin A(6),echiumin B(7),(+)-isolariciresinol-2a-O-β-D-glucopyranoside(8),p-coumaroyl-α-L-rhamnopyranoside(9),p-coumaric acid(10),(-)-5-methoxyisolariciresinol-3α-O-β-D-glucopyranoside(11),rupestrin B(12).Compounds 1-12 were isolated from B.zeylanicum for the first time.(2)Compounds 8 and 12 showed a certain inhibitory effect on the proliferation of SW620 cells,with IC 50 values of(9.34±1.10)μmol·L^(-1)and(0.33±0.06)μmol·L^(-1),respectively.Compound 12 showed a certain inhibitory effect on the proliferation of HT-29 cells,with IC 50 value of(15.94±1.56)μmol·L^(-1).This finding enriches the chemical constituents of B.zeylanicum,and it further clarifies its anti-colorectal cancer activity constituents and which provides a reference for the development and utilization of B.zeylanicum.

关 键 词：鬼点灯 化学成分 结构鉴定 抗结直肠癌活性 乙酸乙酯部 

分 类 号：Q946[生物学—植物学]