西南手参醇提取物的体外抗氧化和体外抗肿瘤活性评价  

Evaluation of in vitro antioxidant and antitumor activity of alcohol extract from Gymnadenia orchidis

作　　者：范晶钰 任雨敏 唐鲜 李玖一 马丹炜[1,2] 聂申明 FAN Jingyu;REN Yumin;TANG Xian;LI Jiuyi;MA Danwei;NIE Shenming(College of Life Sciences,Sichuan Normal University,Chengdu 610101,China;Key Laboratory of Land Resources Evaluation and Monitoring in Southwest China,Ministry of Education(Sichuan Normal University),Chengdu 610066,China;Jiulong County Runde Chinese Herbal Medicine Technology Co.,Ltd.,Jiulong 626200,Sichuan,China)

机构地区：[1]四川师范大学生命科学学院,成都610101 [2]西南土地资源评价与监测教育部重点实验室(四川师范大学),成都610066 [3]四川省九龙县润德中药材科技有限公司,四川九龙626200

出　　处：《广西植物》2025年第2期337-346,共10页Guihaia

基　　金：第四次全国中药资源普查项目(2019PC006);九龙县中藏药资源调查及研究项目(HX20220274);四川省自然科学基金面上项目(23NSFSC0993)。

摘　　要：为挖掘西南手参醇提物的应用潜力,该研究采用高效液相色谱法、生化分析技术和MTT法,对西南手参醇提物的体外抗氧化活性和体外抗肿瘤活性进行了分析。结果表明:(1)供试西南手参醇提物中含有天麻素(质量分数为2.5 mg·g^(-1),浓度为0.25%)。(2)西南手参醇提物对羟基自由基、ABTS+自由基以及DPPH自由基具有显著的清除能力,并随着浓度的升高,作用更加明显,具有浓度依赖性,醇提物的浓度为2.0 mg·mL^(-1)时最为显著,2.0 mg·mL^(-1)的醇提取物对羟基自由基、ABTS+自由基和DPPH自由基的清除率分别为81.68%、19.94%和48.16%;而天麻素仅对羟基自由基具有清除能力,对ABTS+自由基和DPPH自由基没有清除能力。(3)2.0 mg·mL^(-1)的西南手参醇提物对人肝癌SMMC-7721细胞和HepG-2细胞的半数抑制浓度(IC50)分别为5.170 mg·mL^(-1)和4.551 mg·mL^(-1),对人胃癌BGC-823细胞和MKN-45细胞的IC50分别为1.759 mg·mL^(-1)和1.564 mg·mL^(-1),对人乳腺癌MDA-MB-231细胞IC50为2.665 mg·mL^(-1),对人胶质瘤U251细胞的IC50为4.401 mg·mL^(-1),说明西南手参醇提物对人肝癌SMMC-7721细胞和HepG-2细胞、人胃癌BGC-823细胞和MKN-45细胞、人乳腺癌MDA-MB-231细胞以及人胶质瘤U251细胞活性均有显著的抑制作用,其中对人胃癌BGC-823细胞和MKN-45细胞的抑制效果最为显著,表明西南手参的抗肿瘤作用具有广谱性。综上认为,西南手参醇提物具有抗氧化活性,并且比其抗氧化活性物质天麻素具有更广谱的活性氧清除能力,依据活性氧与肿瘤发生之间的关系将其用于抗肿瘤的研究,发现其具有广泛的抗肿瘤特性和天然植物通过多种成分协同作用的特性,可抑制多种肿瘤细胞尤其是人胃癌细胞的增殖。To investigate the potential applications of the alcohol extract from Gymnadenia orchidis,an in vitro study was conducted to evaluate its antioxidant activity and antitumor activity using high-performance liquid chromatography(HPLC),biochemical analysis technology,and the MTT method.The results were as follows:(1)Gastrodin was discovered in the alcohol extract from G.orchidis,with a mass fraction of 2.5 mg·g^(-1).The concentration of gastrodin in the alcohol extract was 0.25%.(2)The alcohol extract from G.orchidis demonstrated a significant scavenging capacity towards hydroxyl radicals,ABTS+radicals,and DPPH radicals.The efficacy of the alcohol extract from G.orchidis becomed more pronounced with an increase in concentration,exhibiting a concentration-dependent response.The alcohol extract from G.orchidis demonstrated the most substantial scavenging capacity at a concentration of 2.0 mg·mL^(-1),the scavenging rates for hydroxyl radical,ABTS+radical,and DPPH radical were 81.68%,19.94%,and 48.16%,respectively.In contrast,gastrodin solely exhibited scavenging activity towards hydroxyl radicals and lacked such activity towards ABTS+and DPPH radicals.(3)The IC 50 of the 2.0 mg·mL^(-1)alcohol extract from G.orchidis on SMMC-7721 and HepG-2 were 5.170 mg·mL^(-1)and 4.551 mg·mL^(-1),respectively.Similarly,the IC 50 of BGC-823 and MKN-45 were 1.759 mg·mL^(-1)and 1.564 mg·mL^(-1),respectively,while the IC 50 of MDA-MB-231 was 2.665 mg·mL^(-1).Furthermore,the IC 50 of U251 was 4.401 mg·mL^(-1).The alcohol extract from G.orchidis demonstrated significant inhibitory effects on SMMC-7721,HepG-2,BGC-823,MKN-45,MDA-MB-231,and U251 cells.Notably,the inhibitory effects on BGC-823 and MKN-45 were the most pronounced,suggesting that the antitumor activity of the alcohol extract from G.orchidis exhibited a wide range of efficacy.The above findings of this research demonstrate that the alcohol extract from G.orchidis possesses antioxidant capabilities,and its ability to scavenge reactive oxygen species is more extensive than

关 键 词：西南手参 天麻素 醇提取物 抗氧化 抗肿瘤 

分 类 号：Q946[生物学—植物学]