炭疽病菌侵染对黄精生理指标及代谢产物的影响  

Effects of Colletotrichum spaethianum Infection on Physiological Indexes and Metabolites in Polygonatum sibiricum

作　　者：宋文佳 公秀萍 徐明轩 束娅菲 张西梅 SONG Wenjia;GONG Xiuping;XU Mingxuan;SHU Yafei;ZHANG Ximei(School of Biology and Brewing Engineering,Taishan University,Taian 271000,China;Medical Research Institute,Southwest University,Chongqing 400715,China;Biotechnology Research Center,Southwest University,Chongqing 400715,China;College of Life Sciences,Shandong Agricultural University,Taian 271018,China;Institute of Medicinal Plants,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100193,China)

机构地区：[1]泰山学院生物与酿酒工程学院,山东泰安271000 [2]西南大学医学研究院,重庆400715 [3]西南大学生物技术中心,重庆400715 [4]山东农业大学生命科学学院,山东泰安271018 [5]中国医学科学院北京协和医学院药用植物研究所,北京100193

出　　处：《中国野生植物资源》2025年第2期61-66,共6页Chinese Wild Plant Resources

基　　金：2021年山东省自然基金(青年)项目(ZR2021QH191);2020年泰山学院引进人才项目(Y-01-2020013);泰安市科技创新发展项目(2023NS120)。

摘　　要：目的:探究炭疽病病原菌侵染对黄精生理指标及代谢产物积累的影响。方法:对黄精根茎进行针刺接种白蜡树炭疽菌(Colletotrichum spaethianum),测量病原菌侵染后盆栽黄精生理指标及代谢产物含量变化。结果:随着C.spaethianum侵染时间的延长,超氧化物歧化酶(SOD)活性和蛋白质含量均明显升高,在监测期间,两者的最高增幅均超过300%,过氧化物酶(POD)活性、丙二醛(MDA)、脂肪和多酚含量均先降低再升高,可溶性糖、多糖及皂苷含量逐渐下降,黄酮含量略有上升。结论:C.spaethianum侵染会影响黄精的生长及代谢,降低黄精多糖、皂苷的含量,影响黄精的药用价值,且会引起植物本身的应激反应,本研究为解析C.spaethianum侵染黄精的生理机制提供了理论基础。Objective:To explore the effects of Colletotrichum spaethianum infection on physiological indexes and metabolite accumulation in Polygonatum sibiricum.Methods:The rhizomes of P.sibiricum were inoculated with C.spaethianum by needle-pricking method,followed by measuring the changes in physiological indexes and metabolites contents of potted P.sibiricum after C.spaethianum infection.Results:As the infection period of C.spaethianum extended,the activities of superoxide dismutase(SOD)and the protein content increased significantly.During the monitoring period,the maximum increase in both exceeded 300%.The activities of peroxidase(POD),as well as the contents of malondialdehyde(MDA),fat and polyphenols,decreased initially and then increased.The contents of soluble sugar,polysaccharides and saponins gradually decreased,while the content of flavonoids content increased slightly.Conclusion:The growth and metabolism of P.sibiricum were affected by C.spaethianum.The contents of polysaccharides and saponins were reduced,thereby impacting the medicinal value of P.sibiricum.Additionally,stress responses in the plant were triggered.This study provided a theoretical basis for understanding the physiological mechanisms of C.spaethianum infection in P.sibiricum.

关 键 词：黄精 白蜡树炭疽菌 生理指标 代谢产物 

分 类 号：Q945.8[农业科学—植物病理学]