基于网络药理学研究鸡血藤对酒精肝的作用机制  

Mechanism of Spatholobus Suberectus Dunn on alcoholic liver based on network pharmacology

作　　者：余浚龙 马威 覃杰湘 兰天莹[1] 黄添 杨洋[1] 陈昌榜 李相玲 YU Junlong;MA Wei;TAN Jiexiang;LAN Tianying;HUANG Tian;YANG Yang;CHEN Changbang;LI Xiangling

机构地区：[1]广西中医药大学,广西南宁530200

出　　处：《中医临床研究》2024年第36期86-92,共7页Clinical Journal Of Chinese Medicine

基　　金：广西中医药大学博士启动基金(2021BS019);广西中医药大学校级课题(2023MS011);广西中医基础研究重点实验室(22-065-54-02);大学生创新创业训练项目(202310600239,S202210600112);本科生科研创新项目(2022DXS19)。

摘　　要：目的:基于网络药理学探讨鸡血藤对酒精肝潜在的作用及发挥调节作用的机制。方法:通过中药系统药理学数据库与分析平台(TCMSP)检索鸡血藤的主要成分,应用Genecard和Disgenet数据库获得酒精性肝病相关靶点,在作图软件Cytoscape 3.9.1中构建鸡血藤有效成分-靶点-疾病网络图,构建鸡血藤调控酒精肝的作用网络;在STRING数据库输入190个鸡血藤与酒精肝的共同靶点,下载相应蛋白互作网络源数据,建立“鸡血藤-酒精肝”靶点蛋白相互作用网络,利用Cytoscape 3.9.1软件得到蛋白质-蛋白质相互作用(PPI)网络图。采用基因本体论(GO)及京都基因与基因组百科全书(KEGG)富集分析主要作用通路,以预测鸡血藤在保护酒精肝方面的潜在机制。结果:鸡血藤中主要含有芦荟大黄素、芒柄花黄素、儿茶素、丙烯酰胺等24个有效成分,可能作用于烟酰胺腺嘌呤二核苷酸磷酸氧化酶4(NOX4)、醛糖还原酶抗体(Aldo-Keto Reductase Family 1 Member B1,AKR1B1)、黄嘌呤脱氢酶(Xanthine Dehydrogenase,XDH)、FMS样酪氨酸激酶3(FMS-like tyrosine ki-nase 3,FLT3)、APP、糖原合成酶激酶3β(Glycogen Synthase Kinase 3 Beta,GSK3B)等190个潜在基因靶点;酪氨酸蛋白激酶(Sarcoma,SRC)、热休克蛋白90α家族A类成员1(Heat Shock Protein 90 Alpha Family Class A Member 1,HSP90AA1)、丝裂原活化蛋白激酶(Mitogen-Activated Protein Kinase,MAPK)1、肿瘤蛋白p53(Tumor Protein p53,TP53)、MAPK3、磷脂酰肌醇3激酶调节亚基1(Phosphoinositide 3-Kinase Regulatory Subunit 1,PIK3R1)、丝氨酸/苏氨酸蛋白激酶1(Akt Serine/Threonine Kinase 1,AKT1)等蛋白靶点,可能通过调控内分泌的阻力、癌症通路、表皮生长因子受体(Epidermal Growth Factor Receptor,EGFR)酪氨酸激酶抑制剂、晚期糖基化终末产物-晚期糖基化终末产物受体(Advanced Glycation End-Products-Receptor Advanced Glycation End Products,AGE-RAGE)信号通路、糖尿病并发症、前列腺癌及磷脂�Objective:To explore the potential mechanism of Spatholobus Suberectus Dunn on alcoholic liver disease based on network pharmacology.Methods:The effective components of Spatholobus Suberectus Dunn were retrieved from Traditional Chinese Medicine Systems Pharmacology(TCMSP)Database and Analysis Platform,and the related targets of alcoholic liver diseases were obtained by using Genecard and Disgenet databases.In the mapping software cytoscape 3.9.1,the active component-targetdisease network of Spatholobus Suberectus Dunn was constructed to construct the functional network of Spatholobus Suberectus Dunn in regulating alcoholic liver.190 common targets of Spatholobus Suberectus Dunn and alcoholic liver were inputted into Staring database,and corresponding protein interaction network source data were downloaded to establish“Spatholobus Suberectus Dunn-alcoholic liver”target protein interaction network by using cytoscape 3.9.1 software,PPI network diagram was obtained.Go and KEGG enrichment analysis were used to predict the potential mechanism of Spatholobus Suberectus Dunn in protecting alcoholic liver.Results:Spatholobus Suberectus Dunn mainly contains 24 effective components such as Aloe Emodin,formononetin,Catechin,acrylamide,etc.,which may be applied to NOX4,AKR1B1,XDH,FLT3,APP,GSK3B and other 190 potential gene targets.SRC,HSP90AA1,MAPK1,TP53,MAPK3,PIK3R1,AKT1 and other protein targets may regulate endocrine resistance,cancer pathways,EGFR tyrosine kinase inhibitors,AGE-RAGE signaling pathway,diabetes complications,prostate cancer,and PI3K-AKT signaling pathway to protect and further treat alcoholic liver.Conclusion:Spatholobus Suberectus Dunn is likely to protect alcoholic liver through its multiple components,multiple targets of alcoholic liver disease and multiple pathways,the PI3K-AKT signaling pathway is likely to be one of the major signaling pathways.This study provides a basis for further study on the mechanism of Spatholobus Suberectus Dunn in liver protection and its subsequent development and utili

关 键 词：鸡血藤 酒精肝 网络药理学 PI3K-AKT信号通路 

分 类 号：R256.4[医药卫生—中医内科学]