基于网络药理学方法研究血府逐瘀汤治疗焦虑障碍的作用机制  

The action mechanism of Xuefu Zhuyu decoction in the treatment of anxiety disorder based on network pharmacology

作　　者：刘宝殊[1] 朱文浩[1] 蒲馨颖 LIU Baoshu;ZHU Wenhao;PU Xinying

机构地区：[1]淄博市中医医院,山东淄博255300

出　　处：《中医临床研究》2024年第30期125-130,共6页Clinical Journal Of Chinese Medicine

摘　　要：目的:运用网络药理学方法研究和探讨血府逐瘀汤治疗焦虑障碍的潜在靶点和作用机制。方法:通过国家中医药百科全书数据库(ETCM)获得血府逐瘀汤中每味中药的归经、成分和靶点,利用Cytoscape软件构建血府逐瘀汤中药归经网络及血府逐瘀汤活性成分-靶点相互作用网络,借助STRING数据库构建蛋白质-蛋白质相互作用(PPI)网络,通过Metascape和David数据库对靶点进行功能富集分析。结果:血府逐瘀汤的中药大多归肝经和心经。血府逐瘀汤作用于肝经、心经,通过疏肝理气、宁心安神治疗焦虑障碍。筛选得到血府逐瘀汤267个有效化合物及673个靶点、药物与疾病共同靶点368个。根据度值大小确定胰岛素(Insulin,INS)、丝氨酸/苏氨酸蛋白激酶1(Akt Serine/Threonine Kinase 1,AKT1)、p-环磷腺苷效应元件结合蛋白(cAMP-response Element Binding Protein,CREB)1、白蛋白(Albumin,ALB)、白细胞介素(Interleukin,IL)6、淀粉样β前体蛋白(Amyloid Beta Precursor Protein,APP)、FOS原癌基因(Fos Proto-Oncogene,FOS)、雌激素受体(Estrogen Receptor,ESR)1等119个关键靶点。基因本体论(GO)富集分析表明血府逐瘀汤治疗焦虑障碍是通过膜电位调节、激素水平的调节、体液调节等生物过程,并涉及γ-氨基丁酸(Gamma-aminobutyric Acid,GABA)门控氯离子通道活性、类固醇羟化酶活性、DNA结合转录因子等分子功能。其作用途径可能与逆行内源性大麻素信号、GABA能突触、肾上腺素信号传导、雌激素信号通路等通路有关。结论:血府逐瘀汤治疗焦虑障碍具有“多成分、多靶点、多通路”的特点,血府逐瘀汤中豆甾醇、β-谷甾醇、槲皮素等成分通过调控内源性大麻素系统、GABA、肾上腺素及雌激素信号通路治疗焦虑障碍。Objective: The potential target and action mechanism of Xuefu Zhuyu decoction( 血府逐瘀汤) in the treatment of anxiety disorder were studied and explored by means of network pharmacology. Methods: The meridian tropisms, composition and target of each traditional Chinese medicine in Xuefu Zhuyu decoction were obtained through ETCM database. The traditional Chinese medicine meridian tropisms network and the interaction network between active ingredients and targets of Xuefu Zhuyu decoction were constructed by Cytoscape software. Protein-protein interaction network was constructed by STRING database. Metascape and David database were used for functional enrichment analysis. Results: The top meridian tropism of traditional Chinese medicine in Xuefu Zhuyu decoction was liver meridian and heart meridian. Xuefu Zhuyu decoction acted on liver meridian and heart meridian, treating anxiety disorders by soothing the liver and regulating Qi( 气), and calming the heart and tranquilizing the mind. A total of 267 effective ingredients and 673 targets of Xuefu Zhuyu decoction were screened, and 368 common targets of drugs and diseases were obtained. According to the degree, 119 key targets such as INS, AKT1, CREB1, ALB, IL-6, APP, FOS and ESR1 were determined. GO enrichment analysis showed that Xuefu Zhuyu decoction treated anxiety disorder by membrane potential regulation, hormone level regulation, body fluid regulation and other biological processes, and involved GABA-gated chloride channel activity, steroid hydroxylase activity, DNA binding transcription factors and other molecular functions. The pathway of action may be related to retrograde endocannabinoid signaling, GABaergic synapses, adrenergic signaling, estrogen signaling and other pathways. Conclusion: Xuefu Zhuyu decoction has the characteristics of ‘multi-ingredient, multitarget and multi-pathway' in the treatment of anxiety disorder. The ingredients of stigasterol, β-sitosterol and quercetin in Xuefu Zhuyu decoction treat anxiety disorder by regulating the

关 键 词：血府逐瘀汤 焦虑障碍 网络药理学 作用机制 

分 类 号：R277.7[医药卫生—中医学]