构树根萌条水培生根发育及解剖观察  

Hydroponic Rooting Development and Anatomical Observation of the Root Sprout of Broussonetia papyrifera

作　　者：刘永贵 梁伟伟 蒋慧儿 欧阳昆唏 周玮 LIU Yong-gui;LIANG Wei-wei;JIANG Hui-er;OU YANG Kun-xi;ZHOU Wei(College of Forestry and Landscape Architecture/South China Agricultural University,Guangdong Provincial Key Laboratory of Forest Plant Germplasm Innovation and Utilization,Guangdong Provincial Engineering Research Center for Woody Feed,Guangzhou,510642,China)

机构地区：[1]华南农业大学林学与风景园林学院,广东省森林植物种质创新与利用重点实验室,广东省木本饲料工程技术研究中心,广东广州510642

出　　处：《山东农业大学学报(自然科学版)》2025年第1期1-10,共10页Journal of Shandong Agricultural University:Natural Science Edition

基　　金：广东省林业科技创新项目(2018KJCX001)。

摘　　要：构树具有重要的生态和经济价值,它不仅可以作为保护环境、修复土壤污染的先锋树种,还可以作为为动物提供蛋白质和能量的畜牧饲料来源。本文采用广州种源构树根萌条为试验材料,设计NAA浓度、IBA浓度、霍格兰浓度和萌条成熟度四因素四水平的正交试验,确定最佳水培处理方式。同时对水培不同时间的萌条生根部位进行石蜡切片,观察不定根形成过程。结果表明,NAA、IBA、霍格兰浓度和成熟度对生根效果指数的影响均极显著;其中,NAA的最佳处理水平为0.1 mg/L,IBA的最佳处理水平为0 mg/L,霍格兰浓度的最佳处理水平为1/4,成熟度的最佳水平为3(保留6个叶片)。通过对不同水培时期的生根萌条基部横切观察,构树萌条水培生根过程可分为三个时期,其中5~6 d为根原基形成时期,9~12 d为不定根的发生时期,12 d以后为不定根的伸长期。构树根萌条水培9 d左右开始出现不定根,不定根原基起源于形成层,为诱生型根原基。成熟度3(保留6个叶片)的构树根萌条在1/4霍格兰+NAA 0.1 mg/L的处理下水培生根效果最佳,生根率达100%,生根效果指数为42.55。Broussonetia papyrifera has important ecological and economic value,can not only be used as an excellent pioneer tree to protect the environment and repair soil pollution,but also used as a high-quality source of livestock feed,providing protein and energy for animals.In this paper,the root sprouts of B.papyrifera of Guangzhou germplasm were used as experimental materials,and orthogonal tests of four factors and four levels of NAA concentration,IBA concentration,Hoagland concentration and sprout maturity were designed to determine the optimal hydroponic treatment method.At the same time,paraffin sections were taken from the root sites of the sprouts with different hydroponic rooting times,to observe the process of adventitious root formation.The results showed that the effects of NAA,IBA,Hoagland concentration and maturity on rooting effect index were very significant.Among them,the optimal treatment level of NAA is 0.1 mg/L,the optimal treatment level of IBA is 0 mg/L,the optimal treatment level of Hoagland concentration is 1/4,and the optimal maturity level is 3(retaining 6 leaves).According to the cross-cutting observation of the root sprout base in different hydroponic periods,the rooting process of the sprout can be divided into three periods,among which the formation period of root primordium on day 5~6,the occurrence period of adventitious roots on day 9~12,and after 12 d is the period of adventitious root elongation.Adventitious roots started to appear around 9 d of hydroponic cultivation of the root sprouting sprouts of B.papyrifera,and the adventitious root primordia originated from the formation layer,which was the induced root primordia.Hydroponic rooting was best when using 1/4 Hoagland+NAA 0.1 mg/L and maturity 3(retaining 6 leaves)root sprouting spike strips of conifers,with 100%rooting rate and rooting effect index of 42.55.

关 键 词：构树 萌条 水培生根 

分 类 号：S722.81[农业科学—林木遗传育种]