丹参白术方“异病同治”冠心病、血管性痴呆、特发性膜性肾病的网络药理学作用机制研究  

Network pharmacological mechanism of Danshen Baizhu prescription on the treatment of coronary heart disease,vascular dementia and idiopathic membranous nephropathy

作　　者：徐璐璐 刘爱军 XU Lulu;LIU Aijun(Department of Pharmacology,School of Pharmacy,Naval Medical University,Shanghai 200433,China;Department of Pharmacy Research,Yueyang Hospital of Integrated Traditional Chinese and Western Medicine,Shanghai University of Traditional Chinese Medicine,Shanghai 200437,China)

机构地区：[1]海军军医大学药学系药理学教研室,上海200433 [2]上海中医药大学附属岳阳中西医结合医院药学研究室,上海200437

出　　处：《药学实践与服务》2025年第3期143-150,共8页Journal of Pharmaceutical Practice and Service

基　　金：国家自然科学基金(82273984);国家重点研发计划(2019YFC1708504);岳阳医院2021院级基金课题(2021yygm01);中医学-中成药临床评价平台(A1-U21-205-902)。

摘　　要：目的基于网络药理学探讨丹参白术方“异病同治”冠心病、血管性痴呆、特发性膜性肾病的物质基础和作用机制。方法通过TCMSP、PubChem、UniProt、GeneCards、OMIM和DrugBank数据库获取药物和疾病靶点,使用STRING数据库和Cytoscape软件绘制中药-化合物-药物靶点网络、复方-共有靶点-疾病网络、蛋白-蛋白互作图,运用Metascape数据库进行基因富集分析。结果共筛选出活性化合物164个,潜在作用靶点509个,“异病同治”的共有靶点141个。其中主要活性成分为丹参酮ⅡA、异鼠李素、槲皮素、木犀草素、山奈酚、β-谷甾醇、豆甾醇等;关键靶点为清蛋白、白介素6、肿瘤坏死因子、丝氨酸/苏氨酸激酶1、血管内皮生长因子A;主要富集于细胞迁移的正向调控、细胞活力的正向调节、蛋白质磷酸化的正向调节、对生长因子的反应、氧化应激反应等生物过程及脂质和动脉粥样硬化、MAPK、动脉粥样硬化和流体剪切力、AGE-RAGE、IL-17、PI3K-Akt等信号通路。结论丹参白术方“异病同治”冠心病、血管性痴呆、特发性膜性肾病的作用机制可能主要通过抑制炎症、抑制氧化应激反应、扩张血管等多靶点、多通路发挥作用。Objective To investigate the material basis and mechanism of Danshen Baizhu prescription in coronary heart disease,vascular dementia and idiopathic membranous nephropathy based on network pharmacology.Methods TCMSP,PubChem,UniProt,GeneCards,OMIM,and DrugBank databases were used to obtain drug and disease targets,and the TCM-compound-drug target network,compound-common target-disease network,and protein-protein interaction map were drawn by STRING database and Cytoscape software,and gene enrichment analysis was performed by Metascape database.Results A total of 164 active compounds,509 potential targets,and 141 common targets were screened out.The main active ingredients were Tanshinone II A,Isorhamnetin,Quercetin,Luteolin,Kampferol,β-sitosterol,Stigmasterol,etc.The key targets were albumin,interleukin 6,Tumor necrosis factor,serine/threonine kinase 1,vascular endothelial growth factor A,mainly enriching in the positive regulation of cell migration,cell viability,protein phosphorylation,responsing to growth factors,oxidative stress and other biological processes and lipid and atherosclerosis,MAPK,atherosclerosis and fluid shear force,AGE-RAGE,IL-17,PI3K-Akt and other signaling pathways.Conclusion The mechanism of action of Danshen Baizhu prescription for coronary heart disease,vascular dementia and idiopathic membranous nephropathy may mainly play a role in multiple targets and pathways such as inhibition of inflammation,inhibition of oxidative stress,and vasodilation.

关 键 词：冠心病 血管性痴呆 特发性膜性肾病 异病同治 网络药理学 

分 类 号：R285.5[医药卫生—中药学]