干旱胁迫训练对党参多糖积累及抗旱性的影响  

Effects of drought stress training on polysaccharide accumulation and drought resistance of Codonopsis pilosula

作　　者：王露露 王晓林 刘喆宇 王利珍 师佳彤 吉姣姣 高建平[1] 白云娥[1] WANG Lu-lu;WANG Xiao-lin;LIU Zhe-yu;WANG Li-zhen;SHI Jia-tong;JI Jiao-jiao;GAO Jian-ping;BAI Yun-e(School of Pharmacy,Shanxi Medical University,Taiyuan 030001,China;Forestry Administration Station,Forestry Bureau of Kelan County,Shanxi Province,Xinzhou 036300,China)

机构地区：[1]山西医科大学药学院,山西太原030001 [2]山西省岢岚县林业局林政站,山西忻州036300

出　　处：《中国中药杂志》2025年第3期672-681,共10页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金青年基金项目(82204569);山西省基础研究计划自然科学研究面上项目(202103021224225);中央本级重大增减支项目(2060302);国家重点研发计划项目(2019YFC1710800)。

摘　　要：为明确干旱胁迫训练对党参品质及抗旱性的影响,该研究采用PEG模拟干旱胁迫和盆栽控水法对党参植株进行干旱胁迫训练,并对干旱胁迫训练后党参多糖含量、次级代谢产物、抗氧化系统、光合色素系统进行分析。结果显示2轮干旱胁迫处理后党参根部果聚糖含量均增加,且显著高于对照组,在复水处理时党参根部果聚糖积累量呈上升趋势;党参炔苷和党参苷Ⅰ含量在干旱胁迫处理后相比于对照组均升高,复水处理使得党参炔苷的含量呈现先升高后降低的趋势,但对党参苷Ⅰ含量变化趋势影响不显著;光合色素含量在干旱胁迫处理后降低,一轮复水和二轮复水过程中含量逐渐升高,且二轮复水升高的较一轮复水快;过氧化产物丙二醛(MDA)含量,超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性在干旱胁迫处理后相比于对照组均升高,在复水过程中呈现降低趋势,且二轮复水降低的较一轮复水得快。经过干旱胁迫训练的党参植株再次受到重度干旱胁迫时植株萎蔫率显著降低,生物量显著增加。该研究表明干旱胁迫训练可以促进党参多糖和党参根系次生代谢产物的积累,当再次遇到干旱胁迫时,党参植株能快速地调节抗氧化系统,延缓叶绿素的分解,提高其抗旱性,为干旱、半干旱地区党参生态种植提供了理论依据。In order to clarify the effects of drought stress training on the quality and drought resistance of Codonopsis pilosula,this study used PEG to simulate drought stress and employed potting with water control for the drought stress training of C.pilosula plants.The polysaccharide content,secondary metabolites,antioxidant system,and photosynthetic pigment system of C.pilosula after drought stress training were analyzed.The results showed that the content of fructans in the root of C.pilosula increased after two rounds of drought stress treatment,and it was significantly higher than that of the control group.The accumulation of fructans in the root of C.pilosula showed an upward trend during the rehydration treatment.The content of lobetyolin and tangshenosideⅠincreased after drought stress treatment compared with that of the control group.The rehydration treatment caused first increasing and then decreasing in the content of lobetyolin,while it had no significant effect on the tangshenosideⅠcontent.The content of photosynthetic pigments decreased after drought stress treatment,and it gradually increased during the first round of rehydration and the second round of rehydration.Moreover,the increase was faster in the second round of rehydration than in the first round of rehydration.The content of the peroxidation product malondialdehyde(MDA)and the activities of superoxide dismutase(SOD),peroxidase(POD),and catalase(CAT)increased after drought stress treatment compared with those of the control group,and they showed a tendency of decreasing during rehydration.Moreover,the decrease was faster in the second round of rehydration than in the first round of rehydration.When the plants of C.pilosula after drought stress training were again subjected to severe drought stress,the wilting rate decreased significantly,and the biomass increases significantly.This study showed that the drought stress training could promote the accumulation of polysaccharides and secondary metabolites in the root of C.pilosula.When encounteri

关 键 词：干旱胁迫训练 党参多糖 次级代谢产物 光合色素 抗氧化系统 抗旱性 

分 类 号：S567.53[农业科学—中草药栽培]