基于气质联用技术的猴头菇多糖抗辐射代谢组学研究  

Anti-radiation metabolomics of Hericium erinaceus polysaccharides based on gas chromatography-mass spectrometry

作　　者：任卓妍 肖炳坤 缪潇瑶 黄荣清 REN Zhuo-yan;XIAO Bing-kun;MIAO Xiao-yao;HUANG Rong-qing(School of Traditional Chinese Medicine,Guangdong Pharmaceutical University,Guangzhou 511436,China;Institute of Radiation Medicine,Academy of Military Medical Sciences,Academy of Military Sciences,Bejing 100850,China)

机构地区：[1]广东药科大学中药学院,广东广州511436 [2]军事科学院军事医学研究院辐射医学研究所,北京100850

出　　处：《中国中药杂志》2025年第3期758-767,共10页China Journal of Chinese Materia Medica

摘　　要：基于气相色谱-质谱联用(GC-MS)的血清代谢组学分析方法探究猴头菇多糖对辐射损伤的代谢调节机制。该实验采用^(60)Co-γ射线照射建立辐射损伤小鼠模型,设计猴头菇多糖注射液高、低剂量组,茜草提取物为阳性对照组,对猴头菇多糖治疗辐射损伤作用及代谢反应途径进行研究。通过GC-MS采集血清样本代谢物,结合主成分分析(PCA)建立各组小鼠的代谢轮廓,并通过偏最小二乘法判别分析(PLS-DA)、t检验(P<0.05)和投影中可变重要性(VIP>1)筛选出差异代谢物;利用HMDB、METLIN等在线数据库进行代谢物的鉴定并构建相关代谢通路和代谢网络。结果表明,在6.5 Gy照射下小鼠血清中有12个差异代谢物与辐射损伤模型有关,分别为乳酸、丙氨酸、尿素、丝氨酸、苏氨酸、甘油、L-5-氧代脯氨酸、L-赖氨酸、软脂酸、硬脂酸、油酸、1-单棕榈糖素;富集了甘油脂代谢和甘氨酸、丝氨酸和苏氨酸代谢这2条代谢通路;8.5 Gy照射下小鼠血清中有18个差异性代谢物与辐射损伤模型相关,分别为乳酸、丙氨酸、尿素、L-亮氨酸、甘油、壬酸、丝氨酸、苏氨酸、L-5-氧代脯氨酸、苯丙氨酸、L-鸟氨酸、1,5-脱氢山梨糖醇、L-赖氨酸、L-酪氨酸、软脂酸、油酸、硬脂酸、胆固醇。富集了苯丙氨酸、酪氨酸和色氨酸合成,苯丙氨酸代谢,甘油酯代谢以及甘氨酸、丝氨酸和苏氨酸代谢这4条代谢通路。提示猴头菇多糖可通过改变小鼠体内氨基酸、脂肪酸合成等途径干预辐射损伤。推测猴头菇多糖是经脂质代谢和氨基酸代谢来调控代谢途径水平,从而影响能量代谢及氨基酸代谢,进而发挥治疗辐射损伤的作用。A serum metabolomics analysis method based on gas chromatography-mass spectrometry(GC-MS)was used to investigate the metabolic regulation mechanism of Hericium erinaceus(H.erinaceus)polysaccharides on radiation injury.A mouse model of radiation injury was established by^(60)Co-γirradiation.High and low dose groups of H.erinaceus polysaccharide injection were designed,and Rubiae Radix et Rhizoma extract was set as the positive control group to investigate the therapeutic effects and metabolic reaction pathways of H.erinaceus polysaccharides on radiation injury.The metabolites of serum samples were collected by GC-MS,and principal component analysis(PCA)was conducted to establish the metabolic profiles of each group of mice.Partial least squares discriminant analysis(PLS-DA),t-test(P<0.05),and variable importance in the projection(VIP>1)were used to screen out the differential metabolite.Metabolite identification and construction of related metabolic pathways and metabolic networks were achieved by using online databases such as HMDB and METLIN.The results showed that 12 differential metabolites in the serum of mice irradiated at 6.5 Gy that were associated with the radiation injury model,including lactic acid,alanine,urea,serine,threonine,glycerol,L-5-oxoproline,L-lysine,stearic acid,stearic acid,oleic acid,and 1-monopalmitoylglucoside.Two metabolic pathways were enriched:glycerolipid metabolism and metabolism of glycine,serine,and threonine.18 differential metabolites in the serum of mice irradiated at 8.5 Gy were associated with the radiation injury model,including lactic acid,alanine,urea,L-leucine,glycerol,nonanoic acid,serine,threonine,L-5-oxoproline,phenylalanine,L-ornithine,1,5-dehydroorbital,L-lysine,L-tyrosine,pectic,oleic,stearic,and cholesterol.Four metabolic pathways were enriched:phenylalanine,tyrosine,and tryptophan synthesis,phenylalanine metabolism,glyceride metabolism,and glycine,serine,and threonine metabolism.It was suggested that H.erinaceus polysaccharides could intervene in radiation injury

关 键 词：猴头菇多糖 抗辐射 气相色谱-质谱联用(GC-MS) 代谢组学 

分 类 号：R285[医药卫生—中药学]