φ26,一株诱导自海南MRSA临床株的溶原性噬菌体  

φ26,a lysogenic phage induced from the methicillin-resistant Staphylococcus aureus in Hainan

作　　者：李艳梅 田申 夏乾峰 LI Yanmei;TIAN Shen;XIA Qianfeng(School of Tropical Medicine,Hainan Medical University,Haikou 571199,China)

机构地区：[1]海南医科大学热带医学院,海南海口571199

出　　处：《海南医科大学学报》2025年第5期321-330,共10页Journal of Hainan Medical University

基　　金：国家自然科学基金资助项目(82260003)。

摘　　要：目的:分离鉴定海南岛耐甲氧西林金黄色葡萄球菌(MRSA)临床株所携带的溶原性噬菌体,确认此类噬菌体的裂解能力,探究其在毒性/抗性基因传播上的能力。方法:收集MRSA临床株,以丝裂霉素C诱导产生噬菌体,经裂解能力比较,筛选得到一株噬菌体φ26,并对其环境稳定性和基因组构成情况进行分析研究。结果:53%的MRSA临床菌株(n=19)可诱导产生噬菌体。其中噬菌体φ26的裂解率最高为95.7%,可在pH=3~11或<60℃的条件下稳定存在。测序确认其基因组为42061 bp线性双链DNA,GC含量34.71%,未检出tRNA,由66个开放阅读框构成,可编码杀白细胞素相关蛋白。结论:本研究获得了一株新型的溶原性MRSA噬菌体,可能介导了临床毒力基因的水平转移,但较高的裂解特性和较好的稳定性提示其与菌体裂解的有关元件存在进一步开发为抗菌试剂的潜力。Objective:To isolate and identify lysogenic phages in clinical isolates of methicillin-resistant Staphylococcus aureus(MRSA)from Hainan Island,determine the lytic ability of these phages,and explore their capacity for spreading virulence/resistance genes.Methods:Lysogenic phages were induced from MRSA clinical isolates using mitomycin C.By assessing their lytic capabilities,a phage,designed asφ26,was subjected to the stability evaluation and the whole genome sequencing.Results:Isolation of lysogenic phages was evidenced in 53%of collected isolates(n=19).Among them,phageφ26 was featured as the highest lytic rate of 95.7%,which was demonstrated to be stable under conditions of pH 3-11 or temperatures below 60℃.Sequencing confirmed that its genome is a 42061 bp linear double-stranded DNA(GC%=34.71%)no tRNA was detected,Including genes encoding leucocidin,a total of 66 open reading frames were identified.Conclusion:A novel lysogenic MRSA phage that may mediate the horizontal transfer of clinical virulence genes was identified in this study,Whilst,its high lytic properties as well as promise stabilities suggested the potential of its lysis associated elements for developing antibacterial agent development in future.

关 键 词：溶原性噬菌体 耐甲氧西林金黄色葡萄球菌 杀白细胞素 

分 类 号：R446.5[医药卫生—诊断学]