柑橘黄龙病干扰甜橙叶片衰老生物学过程的分子机制  

Molecular Mechanism of Huanglongbing Interfering with the BiologicalProcess of Leaf Senescence in Citrus sinensis

作　　者：李瑞民 陈烨[1] 周岩 单思杰 LI Ruimin;CHEN Ye;ZHOU Yan;SHAN Sijie(College of Life Sciences,Gannan Normal University,Ganzhou Jiangxi 341000,China)

机构地区：[1]赣南师范大学生命科学学院,江西赣州341000

出　　处：《植物医学》2025年第1期12-19,共8页Plant Health and Medicine

基　　金：国家自然科学基金(32260659)。

摘　　要：柑橘黄龙病诱导甜橙叶片衰老相关生物学过程的分子机制尚不清楚。为了探索甜橙叶片衰老相关基因在柑橘黄龙病菌侵染过程中的表达模式,利用同源比对方法鉴定甜橙基因组中与叶片衰老相关的基因位点,通过分析前期测定的转录组数据,明确叶片衰老相关基因的表达模式,构建叶片衰老相关蛋白的互作网络,解析叶片衰老相关蛋白的内在联系。结果表明,甜橙基因组中存在108个叶片衰老相关的基因位点,不均匀地分布在9条染色体上,21个甜橙叶片衰老相关基因在柑橘黄龙病菌侵染过程中差异表达,其中9个下调表达,12个上调表达,基因集富集分析发现差异表达基因参与刺激应答,蛋白质互作网络显示甜橙叶片衰老相关蛋白参与细胞自噬、信号转导等生物学过程。研究结果为进一步解析柑橘和黄龙病菌互作过程中的叶片衰老相关基因的生物学功能提供了线索。The molecular mechanisms underlying the induction of leaf senescence in Citrus sinensis by citrus Huanglongbing(HLB)are not yet clear.In order to explore the expression patterns of leaf senescence-related genes in C.sinensis during Candidatus Liberibacter infection,this study used homology alignment methods to identify gene loci related to leaf senescence in the genome of C.sinensis.By analyzing previously obtained transcriptome data,the expression patterns of leaf senescence-related genes of C.sinensis were determined,and an interaction network of leaf senescence-related proteins was constructed to elucidate their intrinsic connections.The results showed that there were 108 leaf senescence-related gene loci in the genome of C.sinensis,unevenly distributed on 9 chromosomes.Among them,21 leaf senescence-related genes exhibited differential expression during Candidatus Liberibacter infection,with 9 down-regulated and 12 up-regulated.Gene set enrichment analysis revealed that differentially expressed genes were involved in stimulus response.The protein interaction network showed that leaf senescence-related proteins in C.sinensis were involved in biological processes such as autophagy and signal transduction.This study provides clues for further understanding the biological functions of leaf senescence-related genes in the interaction between citrus and Candidatus Liberibacter.

关 键 词：甜橙 黄龙病 叶片衰老 生物学过程 富集分析 

分 类 号：S432.4[农业科学—植物病理学]