水杨酸对高盐胁迫下海滨木槿生理特性的缓解效应  

Alleviating Effects of Slicylic Acid on Physiological Characteristics of Hibiscus syriacus L.under High Salt Stress

作　　者：尹娟[1,2] 杨学军 刘伯选 李文杨 Yin Juan;Yang Xuejun;Liu Boxuan;Li Wenyang(Xinyang College of Agriculture and Forestry,Xinyang 464000,China;Xinyang Precision Forestry Application Engineering Technology Research Center,Xinyang 464000,China;State Owned Nursery Farm of Shangcheng County,Shangcheng 465350,China;Administration of Jigongshan National Nature Reserve,Xinyang 464000,China)

机构地区：[1]信阳农林学院,河南信阳464000 [2]信阳市精准林业应用工程技术研究中心,河南信阳464000 [3]商城县国营苗圃场,河南商城465350 [4]鸡公山国家级自然保护区管理局,河南信阳464000

出　　处：《山东农业科学》2025年第2期64-72,共9页Shandong Agricultural Sciences

基　　金：河南省高等学校重点科研项目(19A220005);信阳市科技计划项目(20190038)。

摘　　要：为提高海滨木槿苗的耐盐胁迫能力并为其在高盐渍化土壤中种植提供科学参考,本试验以海滨木槿1年实生苗为材料,分别设置T0(对照:0 NaCl+0 g·L^(-1)水杨酸)、T1(1.0%NaCl+0 g·L^(-1)水杨酸)、T2(0 NaCl+0.3 g·L^(-1)水杨酸)、T3(1.0%NaCl+0.3 g·L^(-1)水杨酸)共4个处理,研究高盐(1.0%NaCl)胁迫下喷施水杨酸对海滨木槿苗生长发育和根系活力、根系渗透调节、根系氧化应激以及根、茎、叶中水杨酸含量的影响。结果表明,高盐胁迫下海滨木槿苗根系受到渗透胁迫,其生长发育迟缓,根系活力降低,细胞氧化应激水平升高;与T1组相比,盐胁迫下喷施水杨酸的T3组植株生长和生物量均显著增加,根系活力以及脯氨酸、可溶性糖、可溶性蛋白含量均显著升高,根系细胞膜透性和丙二醛(MDA)含量则显著降低,同时,在处理第10、20、30天时T3组根系抗氧化酶活性均显著升高,其中,超氧化物歧化酶(SOD)活性分别高出30.98%、30.48%和183.09%,过氧化氢酶(CAT)活性分别高出20.97%、21.32%和192.58%,过氧化物酶(POD)活性分别高出44.06%、42.66%和146.92%。另外,观察到海滨木槿根、茎、叶中水杨酸含量一直处在动态变化中,叶片中含量最高,根系中含量最低。综上,水杨酸具有促进高盐胁迫下海滨木槿生长和提高根系渗透调节和抗氧化的能力,缓解了高盐胁迫对根系造成的损伤。To improve the salt-tolerance of Hibiscus syriacus seedlings and to provide the scientific reference for its planting in highly salinized soil,in this experiment,one-year-old seedlings of H.syriacus were used as materials,and four treatments of T0(0 NaCl+0 g/L salicylic acid),T1(1.0%NaCl+0 g/L salicylic acid),T2(0 NaCl+0.3 g/L salicylic acid)and T3(1.0%NaCl+0.3 g/L salicylic acid)were set.The effects of salicylic acid(SA)application on the growth and development of seedlings,the activity,osmotic adjustment and oxidative stress of roots,and the contents of SA in roots,stems and leaves of H.syriacus under high salt(1.0%NaCl stress)were studied.The results showed that the roots of H.syriacus seedlings were subjected to osmotic stress under high salt stress with the growth and development delayed,root activity decreased,and cellular oxidative stress level increased.Compared with T1 group,the plant growth and biomass in T3 group were increased,the root activity and contents of proline,soluble sugar and soluble protein were significantly increased,while the membrane permeability and malondialdehyde(MDA)content were significantly decreased.Meanwhile,the activity of antioxidant enzymes in roots significantly increased on the 10th,20th,and 30th days after treatment in T3 group,among which,the superoxide dismutase(SOD)activity increased by 30.98%,30.48%and 183.09%;the catalase(CAT)activity increased by 20.97%,21.32%and 192.58%,and the peroxidase(POD)activity increased by 44.06%,42.66%and 146.92%,respectively.In addition,it was observed that the content of SA in the roots,stems and leaves of H.altissima were constantly changing dynamically with the highest in leaves and the lowest in roots.In summary,SA had the ability to promote the growth of H.syriacus and to improve root osmotic regulation and antioxidant capacity under high salt stress,so as to alleviate the damage caused by high salt stress to roots.

关 键 词：水杨酸 盐胁迫 海滨木槿 生理特性 渗透调节 氧化应激 

分 类 号：S792.99[农业科学—林木遗传育种] S685.99[农业科学—林学]