A wheat phytohormone atlas spanning major tissues across the entire life cycle provides novel insights into cytokinin and jasmonic acid interplay  

作　　者：Huanran Yin Wei Liu Xin Hu Jingqi Jia Mengmeng Liu Jiaqi Wei Yikeng Cheng Xin Gongl Qiang Li Wenhao Yan Jizeng Jia Lifeng Gao Alisdair R.Fernie Wei Chen 

机构地区：[1]National Key Laboratory of Crop Genetic Improvement,Hubei Hongshan Laboratory,Huazhong Agricultural University,Wuhan 430070,China [2]State Key Laboratory of Crop Gene Resources and Breeding,National Key Facility for Crop Gene Resources and Genetic Improvement,Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081,China [3]Max-Planck-Institute of Molecular Plant Physiology,14476 Potsdam-Golm,Germany

出　　处：《Molecular Plant》2025年第2期366-382,共17页分子植物(英文版)

基　　金：supported by the National Key Research and Development Program of China(No.2021YFF1000204);the National Key Laboratory of Crop Genetic Improvement Self-Research Program(ZW22A0302 and ZW22B0206);the Natural Science Foundation for Distinguished Young Scientists of Hubei Province(No.2021CFA058),and the Young Topnotch Talent Cultivation Program of Hubei Province。

摘　　要：Although numerous studies have focused on phytohormones in specific organs or tissues at different development stages or under various abiotic and biotic stress conditions,our understanding of the distribution and relative abundance of phytohormones throughout the entire life cycle of plants remains insufficient.Here,we present a phytohormone atlas resource obtained from the quantitative analysis of eight major classes of phytohormones,comprising a total of 40 hormone-related compounds,throughout the complete life cycle of wheat.In combination with transcriptome analysis,we established a wheat phytohormone metabolic regulatory network(WPMRN).Using the WPMRN dataset and Gene Ontology enrichment analysis,we swiftly characterized the function of TaLOG5-B1 in cytokinin biosynthesis.Furthermore,a detailed investigation of the WPMRN dataset uncovered transcription factor-mediated co-regulatory mechanisms among different classes of phytohormones.We focused specifically on the metabolic regulation of cytokinin and jasmonic acid,and functionally characterized the genes TaLOG3-D1 and TaAOS-D1 that are involved in the biosynthesis of these phytohormones,respectively,along with their regulatory transcription factor genes TaDOF3A and TaDOF5.6B.The functions of these genes were validated in transgenic plants,revealing their ability to co-regulate radicle length.These findings serve as a case study that highlights the utility of this resource for studying phytohormone metabolic regulatory networks in cereal crops and for gaining insights into the roles of phytohormones in enhancing agronomic traits.

关 键 词：WHEAT PHYTOHORMONES metabolic regulatory network DOF 

分 类 号：Q94[生物学—植物学]