扛板归多糖对HepG2细胞增殖的体外抑制作用评价  

Evaluation of Antiproliferative Activities of Polysaccharides from Polygonum perfoliatum L.on HepG2 Cells in vitro

作　　者：韦啟球[1,2,3] 王思思 梁柳春 闭爱艳 裴世成 伍善广[1,2,3] 刘雪萍 WEI Qiqiu;WANG Sisi;LIANG Liuchun;BI Aiyan;PEI Shicheng;WU Shanguang;LIU Xueping(Health Science Center,Guangxi University of Science and Technology,Liuzhou 545005,Guangxi,China;Guangxi Key Laboratory of Innovative Research on Medical and Engineering Integration in Colleges and Universities,Liuzhou 545005,Guangxi,China;Liuzhou Key Laboratory of Characteristic Medicinal Resources in Central Guangxi,Liuzhou 545005,Guangxi,China;Pharmaceutical College,Guangxi Medical University,Nanning 530021,Guangxi,China)

机构地区：[1]广西科技大学医学部,广西柳州545005 [2]广西高校医工融合创新研究重点实验室,广西柳州545005 [3]柳州市桂中特色药用资源开发重点实验室,广西柳州545005 [4]广西医科大学药学院,广西南宁530021

出　　处：《食品研究与开发》2025年第5期10-15,37,共7页Food Research and Development

基　　金：国家自然科学基金地区科学基金项目(82160059);广西自然科学基金项目(2020GXNSFAA259025);广西大学生创新创业训练计划项目(202410594023、S202410594025)。

摘　　要：为评价扛板归多糖体外抗肿瘤活性,通过细胞计数试剂盒(cell counting kit⁃8,CCK⁃8)方法测定扛板归多糖对HepG2细胞的体外增殖抑制作用。运用5⁃乙炔基⁃2'⁃脱氧尿嘧啶核苷(5⁃ethynyl⁃2'⁃deoxyuridine,EdU)增殖试验、细胞Hoechst染色试验、线粒体膜电位检测试验、蛋白质免疫印迹试验探究扛板归多糖对肝癌HepG2细胞的抑制作用机制。结果表明:扛板归多糖可以显著抑制HepG2细胞增殖,并呈一定的剂量依赖性,半数抑制浓度为130.81μg/mL。在扛板归多糖作用下,HepG2细胞细胞核固缩、细胞核碎片增加,表现出凋亡特征。扛板归多糖使HepG2细胞线粒体膜电位显著下降;电压依赖性阴离子通道1(voltage⁃dependent anion channel l,VDAC1)、Bax、Cas⁃pase⁃3蛋白表达显著上调,而Bcl⁃2、Mitofusin2蛋白表达下调,均呈现量效相关性。综上,扛板归多糖能够抑制HepG2细胞增殖,可能与影响线粒体功能促进细胞凋亡有关。The antitumor activity in vitro of polysaccharides from Polygonum perfoliatum L.was evaluated.The inhibitory effect of the polysaccharides on the proliferation of HepG2 cells in vitro was evaluated by the cell counting kit⁃8(CCK⁃8)assay.The 5⁃ethynyl⁃2'⁃deoxyuridine(EdU)assay,Hoechst staining,mitochon⁃drial membrane potential assay,and Western blotting were employed to explore the inhibitory mechanism.The results showed that the polysaccharides from P.perfoliatum inhibited the proliferation of HepG2 cells in a con⁃centration⁃dependent manner,with the half maximal inhibitory concentration(IC50)of 130.81μg/mL.After treatment with the polysaccharides,HepG2 cells showed nuclear pycnosis,increased nuclear fragmentation,and apoptosis.The polysaccharides decreased the mitochondrial membrane potential,up⁃regulated the expres⁃sion of voltage⁃dependent anion channel l(VDAC1),Bcl⁃2⁃associated X protein(Bax),and cysteinyl aspar⁃tate⁃specific proteinase⁃3(Caspase⁃3),and down⁃regulated the expression of B⁃cell lymphoma⁃2(Bcl⁃2)and Mitofusin2 in a concentration⁃dependent manner.In conclusion,the polysaccharides from P.perfoliatum inhib⁃ited HepG2 cells by affecting mitochondrial function and promoting cell apoptosis.

关 键 词：扛板归 多糖 HEPG2细胞 体外 抗增殖作用 抗肿瘤活性 

分 类 号：R285[医药卫生—中药学] TS201.4[医药卫生—中医学]